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MAB1569 Anti-Microglia Antibody, clone 25F9

MAB1569
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1569
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Microglia Antibody, clone 25F9
      Alternate Names
      • Macrophages
      • Late State Inflammatory
      Background InformationANTIGEN DISTRIBUTION:

      Isolated cells: Absent on freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 day culture, also positive on some melanoma and carcinoma cell lines. Tissue sections: Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma; synovial lining cells, tuberculoid leprosy; no expression in lepramatous leprosy.
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin from culture supernatant. Lyophilized from PBS, pH 7.2 containing 5 mg/mL BSA and 0.09% sodium azide. Reconstitute with 500 μL of sterile distilled water.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Microglia Antibody, clone 25F9 detects level of Microglia & has been published & validated for use in FC, IC, IH.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:50-1:200 using human tonsil cryosection. The antibody is reactive on formalin fixed / paraffin sections. Enzyme digestion (proteinase pretreatement) is recommended.

      Immunocytochemistry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Clone25F9
      HostMouse
      SpecificityHuman late stage inflammatory macrophages. The monoclonal is suitable for use on BAL (bronchial lavage fluids) and other lavages. The antigen recognized by MAB1569 is an 86 kDa protein (unreduced conditions), probably a glycoprotein on the cell surface and within the cytoplasm of mature macrophages. It is stable to formaldehyde fixation and paraffin embedding. Enzyme digestion is recommended. The antibody has also been reported to recognize microglia stem cells (X International Congress of Neuropathology, Stockholm, le 11 September,1986.PosterA-701).

      Antigen distribution: absent from freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 days in culture, also positive on some melanoma and carcinoma lines. In tissue sections, clone identifies Kupffer cells, histiocytes (skin), macrophages of the thymus, in te germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma;synovial lining cells, tuberculoid leprosy, however no expression in lepramatous leprosy.

      Species reactivity is seen in human, rhesus monkey, and pig, other species not tested.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at 2-8°C for up to 6 months. After reconstitution maintain at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Microglia Antibody, clone 25F9 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Microglia Antibody, clone 25F9 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-MACROPHAGES, LATE STAGE INFLAMMATORY MONOCLONAL ANTIBODY - 2435668 2435668
      MOUSE ANTI-MACROPHAGES, LATE STAGE INFLAMMATORY -MONOCLONAL ANTIBODY 2492316
      MOUSE ANTI-MACROPHAGES, LATE STAGE INFLAMMATORY MONOCLONAL ANTIBODY - 2179348 2179348
      MOUSE ANTI-MACROPHAGES, LATE STAGE INFLAMMATORY MONOCLONAL ANTIBODY - 2325127 2325127
      MOUSE ANTI-MACROPHAGES, LATE STAGE INFLAMMATORY MONOCLONAL ANTIBODY -2492142 2492142