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ABC10 Anti-Megf10 Antibody

Anti-Megf10, Cat. No. ABC10, is a rabbit polyclonal antibody that detects Multiple epidermal growth factor-like domains protein 10 (Mefg10) and is tested for use in Immunofluorescence and Western Blotting.

Anti-Megf10 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: Megf10 UniProt Number: Q6DIB5
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ABC10
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, IFRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC10
DescriptionAnti-Megf10 Antibody
Alternate Names
  • Multiple epidermal growth factor-like domains protein 10
  • Multiple EGF-like domains protein 10
Background InformationMultiple epidermal growth factor-like domains protein 10 (UniProt; Q6DIB5; also known as Multiple EGF-like domains protein 10, Megf10) is encoded by the Megf10 gene (Gene ID: 70417) in murine species. Megf10 is a type I transmembrane membrane receptor protein that is involved in phagocytosis by macrophages and astrocytes of apoptotic cells. Megf10 is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 26-857), a transmembrane protein (aa 858-878), and a cytoplasmic domain (aa 879-1147). It also contains 15 EGF-like domains in the extracellular domain. Its expression is observed in developing myoblasts, muscle stellate cells, stellate cells of the cerebellum, kidney, and macrophages. It is expressed at the cell surface in clusters around cell corpses during engulfment. It serves as a receptor for C1q that binds phosphatidylserine expressed on the surface of apoptotic cells. It is shown to be essential for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum. Megf10 also plays a role in muscle cell proliferation, adhesion, and motility and is shown to be an essential factor in the regulation of myogenesis. It controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway. Mutations in Megf10 gene are reported to cause early onset myopathy, areflexia, respiratory distress, and dysphagia. (Ref.: Saha, M., et al. (2107). Human Mol. Gen. 15(1); 2984-3000).
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • Mouse retina tissue lysate
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Megf10, Cat. No. ABC10, is a rabbit polyclonal antibody that detects Multiple epidermal growth factor-like domains protein 10 (Mefg10) and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesImmunofluorescence Analysis: A representative lot was used to detect Megf10 in P7 caudal brain tissue and retina cells by an independent laboratory. (Image courtesy form the laboratory of Dr. Joshua Sanes, Harvard University).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to the C-terminal, cytoplasmic domain of murine multiple epidermal growth factor-like domains protein 10 (Megf10).
EpitopeCytoplasmic domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects multiple epidermal growth factor-like domains protein 10 (Megf10). It targets an epitope within the cytoplasmic domain.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Megf10
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~130 kDa observed; 122.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse Brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Megf10 in Mouse Brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABC10 04053252550188

Documentation

Anti-Megf10 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Megf10 Antibody Certificates of Analysis

TitleLot Number
Anti-Megf10 2480948
Anti-Megf10 (rabbit polyclonal) - 3062301 3062301
Anti-Megf10 (rabbit polyclonal) - 3523424 3523424
Anti-Megf10 (rabbit polyclonal) - 3523940 3523940
Anti-Megf10 (rabbit polyclonal) - 3677727 3677727
Anti-Megf10 (rabbit polyclonal) - 3755443 3755443
Anti-Megf10 (rabbit polyclonal) - 3821720 3821720
Anti-Megf10 (rabbit polyclonal) - 3841549 3841549
Anti-Megf10 (rabbit polyclonal) - 3852322 3852322
Anti-Megf10 (rabbit polyclonal) - 3892225 3892225
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