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MAB1435 Anti-Macrophages/Monocytes Antibody, clone ED-1

MAB1435
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, BFC, IHC, IP, RIA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1435
      ReplacesCBL1511; CBL260
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Macrophages/Monocytes Antibody, clone ED-1
      Background InformationMonocytes are produced by the bone marrow from haematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They consist of between 3 to 8 percent of the leukocytes in the blood. In the tissues monocytes mature into different types of macrophages at different anatomical locations.
      Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
      References
      Product Information
      FormatPurified
      Control
      • Macrophages, monocytes, lymphoid organs
      PresentationPurified mouse IgG1 in PBS (pH 7.4) and 0.09% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Macrophages/Monocytes Antibody, clone ED-1 is validated for use in Flow Cytometry (FC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), and Western Blotting (WB) for the detection of CD68.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunohistology on frozen sections at 1:50-1:300. Paraffin embedded tissue (Bouin fixation): 1:50-1:300 (requires pretreatment with trypsin or pronase).

      Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.

      Radioimmunoassay

      Also suitable for Immunoprecipitation, and Western Blotting.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRat spleen cells
      CloneED-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes a single chain glycoprotein of 90-100 kD that is expressed on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen has many characteristics in common with macrosialin in the mouse and CD68 in the human.
      Clone ED1, which recognises a CD68 like molecule in rat is an excellent marker of activated microglia. For pan microglial markers, staining both resting and activated microglia, use like CD11b (MAB1387Z and others) or iba-1 (MABN92).
      IsotypeIgG1
      Species Reactivity
      • Rat
      • Bovine
      Species Reactivity NoteBut does not react with horse
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A purified.
      Molecular Weight90-100 kD
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Macrophages/Monocytes Antibody, clone ED-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Macrophages/Monocytes Antibody, clone ED-1 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] MONOCLONAL ANTIBODY - 2121732 2121732
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] MONOCLONAL ANTIBODY - 2426502 2426502
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] MONOCLONAL ANTIBODY - 2449204 2449204
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] - 3172434 3172434
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] - 3209137 3209137
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] - 3251034 3251034
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] - 3277163 3277163
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] - 3313144 3313144
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] - 3420900 3420900
      MOUSE ANTI-RAT MONOCYTES/MACROPHAGES [CD68] - 3466611 3466611