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MAB1407P Anti-Macrophages/Granulocytes Antibody, clone OX-41

MAB1407P
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, ICC, IHC, IH(P), WBMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1407P
      ReplacesMAB1407
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Macrophages/Granulocytes Antibody, clone OX-41
      Alternate Names
      • CD172 antigen-like family member A
      • CD172a antigen
      • Inhibitory receptor SHPS-1
      • Macrophage fusion receptor
      • MyD-1 antigen
      • SHP substrate 1
      • SHP substrate-1
      • Signal-regulatory protein alpha-1
      • signal-regulatory protein alpha
      Background InformationMacrophages and granulocytes express Signal regulatory protein (SIRP), which is a transmembrane glycoprotein involved in the down regulation of tyrosine kinase receptor signal transduction induced by cell adhesion, growth factors or insulin. They are immunoglobulin-like cell surface receptor for CD47. By CD47 binding there is a negative regulation of phagocytosis, mast cell activation and dendritic cell activation that prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Macrophages, monocytes, lymphoid organs.
      PresentationPurified mouse monoclonal IgG2a in buffer containing 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Macrophages/Granulocytes Antibody, clone OX-41 is validated for use in FC, IC, IH, IH(P), WB for the detection of Macrophages/Granulocytes.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      Frozen sections and Formalin fixed, paraffin embedded tissue sections (requires trypsin or pronase pre-treatment).

      Western Blotting:
      A previous lot of this antibody was used in western blot.

      Flow cytometry:
      1:100-1:200 of a previous lot was used. (Use 10 μL of working dilution for 10^6 cells in 100 μL).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRat peritoneal macrophages.
      CloneOX-41
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB1407P recognizes rat SIRP (Signal regulatory protein), which is expressed selectively by myeloid cells and by neurons. This monoclonal binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a and CD47.
      Labels most macrophages including resident peritoneal, alveolar and activated macrophages. Recognition of other macrophages is limited to only occasional kupffer cells. In addition it labels granulocytes and a population of dendritic cells. It also labels brain extensively.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman. Expected to cross-react with rat and mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq]
      Gene Symbol
      • CD172a
      • SIRPA
      • SIRPalpha2
      • BIT
      • SHPS1
      • MFR
      • SIRP
      • p84
      • SHPS-1
      • Sirp-alpha-1
      • CD172A
      • Sirp-alpha-2
      • SIRP-ALPHA-1
      • PTPNS1
      • SIRPalpha
      • P84
      • Bit
      • MYD1
      • MYD-1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P78324 # Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
      SIZE: 503 amino acids; 54813 Da
      SUBUNIT:Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR (By similarity). Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
      PTM: N-glycosylated. & Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
      SIMILARITY: SwissProt: P78324 ## Contains 2 Ig-like C1-type (immunoglobulin-like) domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain
      Molecular Weight56 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Immunocytochemistry on Macrophages/Granulocytes.

      Immunocytochemistry Analysis:
      Confolcal fluorescent analysis of Macrophages/ Granulocytes using anti-Macrophages/ Granulocytes mouse monoclonal antibody.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Macrophages/Granulocytes Antibody, clone OX-41 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Macrophages/Granulocytes Antibody, clone OX-41 Certificates of Analysis

      TitleLot Number
      Anti-Macrophages/Granulocytes, clone -2802052 2802052
      Anti-Macrophages/Granulocytes, clone OX-41 3053815
      Anti-Macrophages/Granulocytes, clone OX-41 2820426
      Anti-Macrophages/Granulocytes, clone OX-41 3314407
      Anti-Macrophages/Granulocytes, clone OX-41 - 3379964 3379964
      Anti-Macrophages/Granulocytes, clone OX-41 - 3455112 3455112
      Anti-Macrophages/Granulocytes, clone OX-41 - 3584418 3584418
      Anti-Macrophages/Granulocytes, clone OX-41 - 3713401 3713401
      Anti-Macrophages/Granulocytes, clone OX-41 - 3843765 3843765
      Anti-Macrophages/Granulocytes, clone OX-41 - 3903302 3903302