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MABF2252-25UG Anti-Macrophage Antibody, clone HAM-56

MABF2252-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2252-25UG
DescriptionAnti-Macrophage Antibody, clone HAM-56
Alternate Names
  • Alveolar macrophages
Background InformationMacrophages are specialized mononuclear phagocytes that are involved in the detection and destruction bacteria and other microorganisms. They also present antigens to T and B-lymphocytes and initiate inflammatory response by releasing cytokines. Macrophages also secrete lysozyme, collagenases, complement components, and coagulation factors. Macrophages are derived from monocytes and display considerable heterogeneity among each macrophage population, which is evident in their morphology, the type of pathogens they recognize, and the levels of inflammatory cytokines they produce. For example, alveolar macrophages phagocytose small particles, dead cells, and bacteria and initiate and control of immunity to respiratory pathogens. They detect bacterial products using their Toll-like receptors. Clone HAM56 recognizes human macrophages, including tangible macrophages found in the germinal center of lymph nodes and tissue macrophages. It is also shown to stain a subpopulation of endothelial cells, most prominently those of the capillaries and smaller blood vessels. However, it does not react with smooth muscle cells, fibroblasts, and with B and T lymphocytes. (Ref.: Gown, AN., et al. (1986). Am. J. Pathol. 125(1); 191-207).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Macrophage, clone HAM-56, Cat. No. MABF2252, is a mouse monoclonal antibody that detects human macrophages and has been tested for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A representative lot detected Macrophage in Immunohistochemistry applications (Gown, A.M., et. al. (1986). Am J Pathol. 125(1):191-207).
Biological Information
ImmunogenHuman alveolar macrophages.
CloneHAM-56
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone HAM-56 is a mouse monoclonal antibody that specifically detects human macrophages.
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Macrophages in human tonsil tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF2252-25UG 04054839007156

Documentation

Anti-Macrophage Antibody, clone HAM-56 Certificates of Analysis

TitleLot Number
Anti-Macrophage, clone HAM-56 - 3245718 3245718

References

Reference overviewPub Med ID
Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions.
Gown, AM; Tsukada, T; Ross, R
Am J Pathol  125  191-207  1986

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