IM1012 Anti-MTA3 (400-450) Rabbit pAb

IM1012
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H Rb Polyclonal Antibody

Pricing & Availability

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IM1012-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the ~62 kDa MTA3 protein in MCF-7 cells.
      Catalogue NumberIM1012
      Brand Family Calbiochem®
      SynonymsAnti-Metastasis-associated gene 3
      Application Data
      Lane 1: Detection of human MTA3 by immunoblotting. Samples: Nuclear extract (25 µg) from MCF-7 cells. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:1000). Detection: chemiluminescence. Lanes 2 and 3: Detection of human MTA3 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (500 µg) (lane 2). Antibody for IP: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:100) and immunoprecipitation control (lane 3): nonspecific rabbit IgG (1:100). Immunoblotting conditions: same as lane 1. Detection: chemiluminescence.

      Detection of human MTA3 by immunocytochemistry. Sample: Sk-Mel-28 cells fixed for 5 min with ice cold methanol and blocked with normal goat serum. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:300). Detection: fluorescence.
      References
      ReferencesFujita, N., et. al. 2003. Cell 113, 207.
      Kumar R. 2003. Cell 113, 142.
      Simpson, A, et.al. 2001. Gene 273, 29.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlMCF7 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1:500-1:5000)
      Immunocytochemistry (1:300)
      Immunoprecipitation (2-10 µg/mg lysate)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids within residues 400-450 of human MTA3
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MTA3 (400-450) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MTA3 (400-450) Rabbit pAb Certificates of Analysis

      TitleLot Number
      IM1012

      References

      Reference overview
      Fujita, N., et. al. 2003. Cell 113, 207.
      Kumar R. 2003. Cell 113, 142.
      Simpson, A, et.al. 2001. Gene 273, 29.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-Metastasis-associated gene 3
      ApplicationImmunoblotting (1:500-1:5000)
      Immunocytochemistry (1:300)
      Immunoprecipitation (2-10 µg/mg lysate)
      Application Data
      Lane 1: Detection of human MTA3 by immunoblotting. Samples: Nuclear extract (25 µg) from MCF-7 cells. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:1000). Detection: chemiluminescence. Lanes 2 and 3: Detection of human MTA3 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (500 µg) (lane 2). Antibody for IP: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:100) and immunoprecipitation control (lane 3): nonspecific rabbit IgG (1:100). Immunoblotting conditions: same as lane 1. Detection: chemiluminescence.

      Detection of human MTA3 by immunocytochemistry. Sample: Sk-Mel-28 cells fixed for 5 min with ice cold methanol and blocked with normal goat serum. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:300). Detection: fluorescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~62 kDa MTA3 protein.
      BackgroundMTA1, MTA2, and MTA3 (Metastasis-associated gene 3, ACC# AAH53631) are members of a growing novel gene family associated with highly metastatic human carcinomas. MTA3 is expressed in all tissues as both 515 aa (MTA3) and 594 aa (MTA3L) proteins found in both the nucleus and the cytoplasmic compartments. It was identified as an Estrogen receptor (ER) regulated component of the Mi/NuRD histone deacetylase transcriptional repressor. In response to estrogen, MTA3 directly targets Snail, the master regulator of the epithelial to mesenchymal transition (EMT). Regulation of E-cadherin expression due to MTA3 and ER mediated signaling of Snail expression may be an important pathway in human breast tumors. Loss of ER results in decreased MTA3 expression and tamoxifen reduces expression of MTA3 in endometrial cells.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids within residues 400-450 of human MTA3
      IsotypeIgG
      Specieshuman
      Positive controlMCF7 cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
      Toxicity Highly Toxic
      ReferencesFujita, N., et. al. 2003. Cell 113, 207.
      Kumar R. 2003. Cell 113, 142.
      Simpson, A, et.al. 2001. Gene 273, 29.