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OP145 Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
OP145-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~90 kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.
Catalogue NumberOP145
Brand Family Calbiochem®
SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
Application Data
Detection of human MDM2 by immunocytochemistry. Sample: MCF7 cells. Primary antibody: Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) (Cat. No. OP145) (2.5 µg/ml). Detection: fluorescence.
References
ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO. J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J.D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J.D., et al. 1992. Nature 358, 80.
Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.
Positive controlA549 or MCF7 cells or breast carcinoma tissue
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmunoprecipitation Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (2 µg/ml, chemiluminescence)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/reaction, see application references)
Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human MDM2
ImmunogenHuman
Clone4B2C1.11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
OP145-100UG 04055977224627

Documentation

Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) SDS

Title

Safety Data Sheet (SDS) 

Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) Certificates of Analysis

TitleLot Number
OP145

References

Reference overview
Marchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO. J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J.D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J.D., et al. 1992. Nature 358, 80.
Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-June-2011 RFH
SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
ApplicationImmunoblotting (2 µg/ml, chemiluminescence)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/reaction, see application references)
Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
Application Data
Detection of human MDM2 by immunocytochemistry. Sample: MCF7 cells. Primary antibody: Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) (Cat. No. OP145) (2.5 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.
BackgroundIncreased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events. Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extrachromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH3T3 and Rat2 cells. The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas. Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation. MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators. MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators. A functional role for MDM2 has recently been identified on the basis of experiments which demonstrate that MDM2 forms a stable complex with p53 in vivo. Furthermore, over-expression of MDM2 inhibits the ability of wild type p53 to stimulate expression of target genes and this inhibition appears to result from MDM2 binding to the acidic activation domain of p53, thereby preventing p53 from directly contacting the transcriptional machinery. Interestingly, wild type p53 appears to positively regulate expression of the MDM2 gene. MDM2 amplification has been observed in sarcomas and direct analysis of the MDM2 and p53 genes in sarcomas indicates that one or the other but not both of these genes is mutated in 70% of the tumors. Thus, it appears that genetic alterations in either p53 or MDM2 represent alternative mechanisms for inactivating the same growth suppressive pathway. Regulation of MDM2 expression by p53 also represents a potential feed back control of p53 function.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human MDM2
Clone4B2C1.11
IsotypeIgG₁
Specieshuman
Positive controlA549 or MCF7 cells or breast carcinoma tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO. J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J.D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J.D., et al. 1992. Nature 358, 80.
Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.
Application referencesImmunoprecipitation Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.