434636 Anti-Lewis y Mouse mAb (F3)

434636
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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434636-1ML
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      Plastic ampoule 1 ml
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      Description
      OverviewRecognizes the Lewis y carbohydrate chain in colon adenocarcinoma.
      Catalogue Number434636
      Brand Family Calbiochem®
      SynonymsAnti-Ley
      References
      ReferencesCordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
      Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
      Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
      Product Information
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Positive controlColon adenocarcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunohistochemistry
      Application NotesImmunohistochemistry (1:10-1:120)
      Application CommentsFor immunohistochemistry, fix tissue in 10% formalin buffered to neutral pH. Variables associated with assay conditions will dictate the proper working dilutions.
      Biological Information
      Immunogenhuman SK-LU-3 lung cancer cells
      ImmunogenHuman
      CloneF3
      HostMouse
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Lewis y Mouse mAb (F3) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Lewis y Mouse mAb (F3) Certificates of Analysis

      TitleLot Number
      434636

      References

      Reference overview
      Cordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
      Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
      Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      SynonymsAnti-Ley
      ApplicationImmunohistochemistry (1:10-1:120)
      DescriptionPurified mouse monoclonal antibody. Recognizes the Lewis y blood group antigen.
      BackgroundBlood group antigens are a group of molecules formed by sequential addition of saccharides to carbohydrate side chains of lipids and proteins and are detected on erythrocytes, certain epithelial cells and in secretions of some individuals. A, B and H antigens are synthesized by erythrocytes and integrated into their cell membranes. Lewis antigens are not synthesized by erythrocytes but can be absorbed from the plasma onto the surface of red blood cells. Subtle differences in the composition and arrangement of the sugars in the chains are sufficient to generate these distinct specificities. Lewis y is often detected in colorectal carcinomas and in renal, bladder and large-bowel tumors.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman SK-LU-3 lung cancer cells
      CloneF3
      IsotypeIgM
      Specieshuman
      Positive controlColon adenocarcinoma tissue
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor immunohistochemistry, fix tissue in 10% formalin buffered to neutral pH. Variables associated with assay conditions will dictate the proper working dilutions.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCordon-Cardo, C., et al. 1986. Int. J. Cancer 37, 667.
      Sakamoto, J., et al. 1986. Cancer Res. 46, 1553.
      Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.