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07-432 Anti-K+/Cl- Cotransporter (KCC2) Antibody

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07-432
100 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, CaICC, WBRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-432
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-K+/Cl- Cotransporter (KCC2) Antibody
Alternate Names
  • Electroneutral potassium-chloride cotransporter 2
  • Erythroid K-Cl cotransporter 2
  • Neuronal K-Cl cotransporter
  • electroneural potassium-chloride cotransporter 2
  • solute carrier family 12 (potassium-chloride transporter), member 5
Background InformationKCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Rat brain membrane preparation.
PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
Quality LevelMQ100
Applications
ApplicationThis Anti-K+/Cl-Cotransporter (KCC2) Antibody is validated for use in IC, WB for the detection of K+/Cl-Cotransporter (KCC2) with more than 15 product citations.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry:
This antibody has been reported by an independent laboratory to detect K+/Cl- Cotransporter (KCC2) in Madin Darby Canine Kidney (MDCK) cells at 1:200 dilution.
Biological Information
ImmunogenN-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140 kDa. An unknown band >200 kDa was also detected.
IsotypeIgG
Species Reactivity
  • Rat
  • Canine
Species Reactivity NoteRat and dog. Wide range of species cross-reactivity expected due to sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryK-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons.
Gene Symbol
  • SLC12A5
  • KIAA1176
  • hKCC2
  • KCC2
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9H2X9 # Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
SIZE: 1139 amino acids; 126184 Da
SUBUNIT: Homomultimer and heteromultimer with other K-Cl cotransporters (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain specific. Detected in neuronal cells.
SIMILARITY:SwissProt: Q9H2X9 ## Belongs to the SLC12A transporter family.
MISCELLANEOUS: Inhibited by furosemide and bumetanide.
Molecular Weight140 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot in rat brain membrane preperations.

Western Blot Analysis: 1.0 µg/mL of this lot detected K+/Cl- Cotransporter (KCC2) in rat brain membrane preparations.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-432 04053252580659

Documentation

Anti-K+/Cl- Cotransporter (KCC2) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-K+/Cl- Cotransporter (KCC2) Antibody Certificates of Analysis

TitleLot Number
Anti-K+/Cl- Cotransporter (KCC2) - 2120128 2120128
Anti-K+/Cl- Cotransporter (KCC2) - 2373389 2373389
Anti-K+/Cl- Cotransporter (KCC2) - 2452523 2452523
Anti-K+/Cl- Cotransporter (KCC2) - 1959267 1959267
Anti-K+/Cl- Cotransporter (KCC2) - 2019601 2019601
Anti-K+/Cl- Cotransporter (KCC2) - 2068566 2068566
Anti-K+/Cl- Cotransporter (KCC2) - 2193161 2193161
Anti-K+/Cl- Cotransporter (KCC2) - 2280419 2280419
Anti-K+/Cl- Cotransporter (KCC2) - 2465197 2465197
Anti-K+/Cl- Cotransporter (KCC2) - 2730897A 2730897A

References

Reference overviewApplicationSpeciesPub Med ID
Early depolarizing GABA controls critical-period plasticity in the rat visual cortex.
Deidda, G; Allegra, M; Cerri, C; Naskar, S; Bony, G; Zunino, G; Bozzi, Y; Caleo, M; Cancedda, L
Nature neuroscience  18  87-96  2015

Show Abstract
25485756 25485756
Age- and sex-dependent susceptibility to phenobarbital-resistant neonatal seizures: role of chloride co-transporters.
Kang, SK; Markowitz, GJ; Kim, ST; Johnston, MV; Kadam, SD
Frontiers in cellular neuroscience  9  173  2015

Show Abstract
26029047 26029047
Synaptic elements for GABAergic feed-forward signaling between HII horizontal cells and blue cone bipolar cells are enriched beneath primate S-cones.
Puller, C; Haverkamp, S; Neitz, M; Neitz, J
PloS one  9  e88963  2014

Show Abstract
24586460 24586460
Gephyrin clusters are absent from small diameter primary afferent terminals despite the presence of GABA(A) receptors.
Lorenzo, LE; Godin, AG; Wang, F; St-Louis, M; Carbonetto, S; Wiseman, PW; Ribeiro-da-Silva, A; De Koninck, Y
The Journal of neuroscience : the official journal of the Society for Neuroscience  34  8300-17  2014

Show Abstract
24920633 24920633
Down-regulation of KCC2 expression and phosphorylation in motoneurons, and increases the number of in primary afferent projections to motoneurons in mice with post-stroke spasticity.
Toda, T; Ishida, K; Kiyama, H; Yamashita, T; Lee, S
PloS one  9  e114328  2014

Show Abstract
25546454 25546454
Nitric oxide signaling modulates synaptic inhibition in the superior paraolivary nucleus (SPN) via cGMP-dependent suppression of KCC2.
Yassin, L; Radtke-Schuller, S; Asraf, H; Grothe, B; Hershfinkel, M; Forsythe, ID; Kopp-Scheinpflug, C
Frontiers in neural circuits  8  65  2014

Show Abstract
Immunofluorescence24987336 24987336
Overexpression of BDNF increases excitability of the lumbar spinal network and leads to robust early locomotor recovery in completely spinalized rats.
Ziemlińska, E; Kügler, S; Schachner, M; Wewiór, I; Czarkowska-Bauch, J; Skup, M
PloS one  9  e88833  2014

Show Abstract
24551172 24551172
An unexpected role of neuroligin-2 in regulating KCC2 and GABA functional switch.
Sun, C; Zhang, L; Chen, G
Molecular brain  6  23  2013

Show Abstract
23663753 23663753
Immunolocalization of cation-chloride cotransporters in the developing and mature spinal cord of opossums, Monodelphis domestica.
Phan, HL; Pflieger, JF
Frontiers in neuroanatomy  7  12  2013

Show Abstract
23720613 23720613
Decreased Immunoreactivities of the Chloride Transporters, KCC2 and NKCC1, in the Lateral Superior Olive Neurons of Kanamycin-treated Rats.
Suh, MW; Ahn, SC
Clinical and experimental otorhinolaryngology  5  117-21  2012

Show Abstract
22977707 22977707