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MAB1961Z Anti-Integrin αVβ5 Antibody, clone P1F6, azide free

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MAB1961Z
100 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, HFC, IHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1961Z
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin αVβ5 Antibody, clone P1F6, azide free
Alternate Names
  • MAB1961
Background InformationThe product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
References
Product Information
FormatPurified
Control
  • Epithelium, keratinocytes
PresentationPurified immunoglobulin in 0.01M phosphate buffer, pH 7.1, 0.15M NaCl containing no preservatives.
Quality LevelMQ100
Applications
ApplicationDetect Integrin αVβ5 using this Anti-Integrin αVβ5 Antibody, clone P1F6, azide free validated for use in FC, IH.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Affects Function
Applications Not Recommended
  • Immunohistochemistry (Paraffin)
Application NotesInhibition of attachment of HT29 cells to vitronectin: >1:1,000

Inhibition of attachment of carcinoma and melanoma cells to vitronectin

Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue. (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.)

Immunocytochemistry: for use on human carcinomas, normal epithelium and keratinocytes 1:1500 (Acetone Fixed Only)

Immunoprecipitation

FACS

EIA

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman UCLA P3 cells (lung carcinoma)
CloneP1F6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the human integrin alphaVbeta5.
IsotypeIgG1
Species Reactivity
  • Chicken
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
Gene Symbol
  • ITGAV
  • DKFZp686A08142
  • MSK8
  • CD51
  • VNRA
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB1961Z 04053252501524

Documentation

Anti-Integrin αVβ5 Antibody, clone P1F6, azide free SDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin αVβ5 Antibody, clone P1F6, azide free Certificates of Analysis

TitleLot Number
MOUSE ANTI-HUMAN INTEGRIN #945;V#946;5 PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2074048 2074048
MOUSE ANTI-HUMAN INTEGRIN aVB5 PRESERVATIVE FREE MONOCLONAL ANTIBODY 2918939
MOUSE ANTI-HUMAN INTEGRIN aVb5 PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2289144 2289144
MOUSE ANTI-HUMAN INTEGRIN αVβ5 PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2428686 2428686
MOUSE ANTI-HUMAN INTEGRIN αVβ5 PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2465271 2465271
MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3237513 3237513
MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3433648 3433648
MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3683816 3683816
MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3774201 3774201
MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3863388 3863388

References

Reference overviewApplicationPub Med ID
Isthmin targets cell-surface GRP78 and triggers apoptosis via induction of mitochondrial dysfunction.
Chen, M; Zhang, Y; Yu, VC; Chong, YS; Yoshioka, T; Ge, R
Cell death and differentiation  21  797-810  2014

Show Abstract
24464222 24464222
New blocking antibodies impede adhesion, migration and survival of ovarian cancer cells, highlighting MFGE8 as a potential therapeutic target of human ovarian carcinoma.
Tibaldi, L; Leyman, S; Nicolas, A; Notebaert, S; Dewulf, M; Ngo, TH; Zuany-Amorim, C; Amzallag, N; Bernard-Pierrot, I; Sastre-Garau, X; Théry, C
PloS one  8  e72708  2013

Show Abstract
Fluorescence Activated Cell Sorting (FACS)23977342 23977342
Synstatin: a selective inhibitor of the syndecan-1-coupled IGF1R-αvβ3 integrin complex in tumorigenesis and angiogenesis.
Rapraeger, Alan C
FEBS J., (2013)  2013

Show Abstract
23375101 23375101
Dual inhibition of αV integrins and Src kinase activity as a combination therapy strategy for colorectal cancer.
Jia, J; Starodub, A; Cushman, I; Liu, Y; Marshall, DJ; Hurwitz, HI; Nixon, AB
Anti-cancer drugs  24  237-50  2013

Show Abstract
23275294 23275294
Assessment of the integrin alpha5beta1 antagonist JSM6427 in proliferative vitreoretinopathy using in vitro assays and a rabbit model of retinal detachment.
Zahn, G; Volk, K; Lewis, GP; Vossmeyer, D; Stragies, R; Heier, JS; Daniel, PE; Adamis, AP; Chapin, EA; Fisher, SK; Holz, FG; Löffler, KU; Knolle, J
Investigative ophthalmology & visual science  51  1028-35  2010

Show Abstract
19815730 19815730
Fluctuations of cytoskeleton-bound microbeads--the effect of bead-receptor binding dynamics.
C Metzner,C Raupach,C T Mierke,B Fabry
Journal of physics. Condensed matter : an Institute of Physics journal  22  2010

Show Abstract
21386432 21386432
Integrin alphaVbeta6 is a high-affinity receptor for coxsackievirus A9.
Heikkilä, O; Susi, P; Stanway, G; Hyypiä, T
The Journal of general virology  90  197-204  2009

Show Abstract
19088289 19088289
Src, PKCalpha, and PKCdelta are required for alphavbeta3 integrin-mediated metastatic melanoma invasion.
Putnam, Andrew J, et al.
Cell Commun. Signal, 7: 10 (2009)  2009

Show Abstract
19400942 19400942
Endothelial cell lumen and vascular guidance tunnel formation requires MT1-MMP-dependent proteolysis in 3-dimensional collagen matrices.
Stratman, AN; Saunders, WB; Sacharidou, A; Koh, W; Fisher, KE; Zawieja, DC; Davis, MJ; Davis, GE
Blood  114  237-47  2009

Show Abstract Full Text Article
19339693 19339693
Anti-integrin monoclonal antibody CNTO 95 enhances the therapeutic efficacy of fractionated radiation therapy in vivo.
Ning, S; Nemeth, JA; Hanson, RL; Forsythe, K; Knox, SJ
Molecular cancer therapeutics  7  1569-78  2008

Show Abstract
18566227 18566227

Data Sheet

Title
MOUSE ANTI-HUMAN INTEGRIN alphaVbeta5 PRESERVATIVE FREE

Related Products & Applications

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Alternative Format

Catalog Number Description
MAB1961 Anti-Integrin αVβ5 Antibody, clone P1F6
MAB1961F Anti-Integrin αVβ5 Antibody, clone P1F6, FITC conjugated

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies