Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MAB1953Z Anti-Integrin αV Antibody, clone P3G8, azide free

View Products on Sigmaaldrich.com
MAB1953Z
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, IP, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1953Z
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin αV Antibody, clone P3G8, azide free
Alternate Names
  • CD51
  • MAB1953
References
Product Information
FormatPurified
Quality LevelMQ100
Applications
ApplicationThis Anti-Integrin αV Antibody, clone P3G8, azide free is validated for use in ELISA, FC, IP, IC, IH for the detection of Integrin αV.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunohistochemistry. For use on acetone or paraformaldehyde fixed tissue

Immunocytochemistry. Will react with some lymphoid cell lines (B cells), many carcinoma and melanoma cell lines and osteosarcomas.

Flow cytometry

Immunoprecipitation

EIA

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenUCLA P3 lung carcinoma cells
CloneP3G8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with human alphaV integrin subunit and with all alphaV-containing integrin receptors.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
Gene Symbol
  • ITGAV
  • DKFZp686A08142
  • MSK8
  • CD51
  • VNRA
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB1953Z 04053252578076

Documentation

Anti-Integrin αV Antibody, clone P3G8, azide free SDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin αV Antibody, clone P3G8, azide free Certificates of Analysis

TitleLot Number
MOUSE ANTI-HUMAN INTEGRIN #945;V PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2135123 2135123
MOUSE ANTI-HUMAN INTEGRIN aV PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2031338 2031338
MOUSE ANTI-HUMAN INTEGRIN aV PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2044295 2044295
MOUSE ANTI-HUMAN INTEGRIN aV PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2106903 2106903
MOUSE ANTI-HUMAN INTEGRIN aV PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2298368 2298368
MOUSE ANTI-HUMAN INTEGRIN αV PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2394020 2394020
MOUSE ANTI-HUMAN INTEGRIN αV - 3817581 3817581
MOUSE ANTI-HUMAN INTEGRIN αV - 3847533 3847533
MOUSE ANTI-HUMAN INTEGRIN αV - 3949244 3949244
MOUSE ANTI-HUMAN INTEGRIN αV - 4030992 4030992

References

Reference overviewPub Med ID
Dual inhibition of αV integrins and Src kinase activity as a combination therapy strategy for colorectal cancer.
Jia, J; Starodub, A; Cushman, I; Liu, Y; Marshall, DJ; Hurwitz, HI; Nixon, AB
Anti-cancer drugs  24  237-50  2013

Show Abstract
23275294 23275294
Neisseria gonorrhoeae-induced transactivation of EGFR enhances gonococcal invasion.
Swanson KV, Griffiss JM, Edwards VL, Stein DC, Song W.
Cellular microbiology  13  1078-90  2011

Show Abstract
21501367 21501367
Neisseria meningitidis adhesin NadA targets beta1 integrins: functional similarity to Yersinia invasin.
Nägele, V; Heesemann, J; Schielke, S; Jiménez-Soto, LF; Kurzai, O; Ackermann, N
The Journal of biological chemistry  286  20536-46  2011

Show Abstract
21471204 21471204
Endothelial cell lumen and vascular guidance tunnel formation requires MT1-MMP-dependent proteolysis in 3-dimensional collagen matrices.
Stratman, AN; Saunders, WB; Sacharidou, A; Koh, W; Fisher, KE; Zawieja, DC; Davis, MJ; Davis, GE
Blood  114  237-47  2009

Show Abstract Full Text Article
19339693 19339693
Design and activity of multifunctional fibrils using receptor-specific small peptides.
Y Ohga, F Katagiri, K Takeyama, K Hozumi, Y Kikkawa, N Nishi, M Nomizu
Biomaterials  30  6731-8  2009

Show Abstract
19765823 19765823
PCI-enhanced adenoviral transduction employs the known uptake mechanism of adenoviral particles.
Engesaeter, BØ; Bonsted, A; Berg, K; Høgset, A; Engebråten, O; Fodstad, Ø; Curiel, DT; Maelandsmo, GM
Cancer gene therapy  12  439-48  2005

Show Abstract
15678152 15678152
Novel compound enables high-level adenovirus transduction in the absence of an adenovirus-specific receptor.
Christine M Fouletier-Dilling, Pablo Bosch, Alan R Davis, Jessica A Shafer, Steven L Stice, Zbigniew Gugala, Francis H Gannon, Elizabeth A Olmsted-Davis
Human gene therapy  16  1287-97  2005

Show Abstract
16259562 16259562
The anchoring protein RACK1 links protein kinase Cepsilon to integrin beta chains. Requirements for adhesion and motility.
Besson, A; Wilson, TL; Yong, VW
The Journal of biological chemistry  277  22073-84  2002

Show Abstract
11934885 11934885
Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases.
Kapila, Y L, et al.
J. Biol. Chem., 274: 30906-13 (1999)  1999

Show Abstract
10521484 10521484
Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor.
Weinacker, A, et al.
J. Biol. Chem., 269: 6940-8 (1994)  1994

Show Abstract
8120056 8120056

Related Products & Applications

Related Products

Alternative Format

Catalog Number Description
MAB1953 Anti-Integrin αV Antibody, clone P3G8

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies