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MAB2058 Anti-Integrin β4 Antibody, clone ASC-3

MAB2058
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, IHC, FUNCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2058
      Replaces05-283
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β4 Antibody, clone ASC-3
      Alternate Names
      • CD104
      References
      Product Information
      FormatPurified
      PresentationLiquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Integrin β4 using this Anti-Integrin β4 Antibody, clone ASC-3 validated for use in FC, IP, IH.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry
      • Affects Function
      Application NotesInhibition of cell attachment: inhibits SCC9 cell adhesion to human

      laminin and laminin GD-2 peptide, but not to EHS laminin, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.

      Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.

      Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

      Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells.

      Working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman squamous cell carcinoma cells (SCC9)
      CloneASC-3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityHuman beta4 integrin (CD104)
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • ITGB4
      • CD104
      • GP150
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16144 # Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
      SIZE: 1822 amino acids; 202151 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
      DOMAIN: SwissProt: P16144 The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
      DISEASE: SwissProt: P16144 # Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. & Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. This nonlethal form of junctional epidermolysis bullosa is characterized by life- long blistering of the skin, associated with hair and tooth abnormalities.
      SIMILARITY: Belongs to the integrin beta chain family. & Contains 1 Calx-beta domain. & Contains 4 fibronectin type-III domains. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Integrin β4 Antibody, clone ASC-3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin β4 Antibody, clone ASC-3 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN B4 [CD104] MONOCLONAL ANTIBODY 2964464
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] 3074355
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] - 2540390 2540390
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] - 3437950 3437950
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2517348 2517348
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2581711 2581711
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2649633 2649633
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2669943 2669943
      MOUSE ANTI-HUMAN INTEGRIN β4 [CD104], MONOCLONAL ANTIBODY 2834090
      MOUSE ANTI-HUMAN INTEGRIN 4 [CD104] -2567747 2567747

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      Alternative Format

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies