Millipore Sigma Vibrant Logo

AB1922 Anti-Integrin β4 Antibody

AB1922
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB1922
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β4 Antibody
      Alternate Names
      • CD104
      References
      Product Information
      FormatSerum
      PresentationWhole rabbit serum. Liquid, with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Integrin β4 Antibody is an antibody against Integrin β4 for use in IP, IH.
      Key Applications
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesImmunoprecipitation from labeled cell extracts: 5 μL of serum is sufficient to precipitate alpha6beta4 from 1-2 x 10E6 cells. In some cases immunoprecipitation from 35S methionine labeled cells results in two bands of 95 and 55 kDa of unknown origin in addition to the 200 kDa and 140 kDa bands corresponding to beta4 and alpha6.

      Immunohistochemistry: 5 μg/mL. See protocol below.

      Optimal working dilutions must be determined by the end user.



      Suggested Protocol for Immunohistochemical:

      1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.

      2. Air Dry.

      3. Wash with 2 mL of 1x PBS.

      4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.

      5. Wash with 5 mL of 1x PBS.

      6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber

      7. Wash with 5 mL of 1x PBS.

      8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.

      9. Block the reaction by washing with 2 mL of 1x PBS.

      10. Counterstain with hematoxylin as desired.
      Biological Information
      ImmunogenSynthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human β4 integrin subunit (Tamura et al. 1990). The sequence used is NH2-(K)GTLSTHMDQQFFQT-amide. The lysine (K) is not in the β4 sequence and was added to conjugate the peptid
      HostRabbit
      SpecificitySpecific for beta4 integrin as determined by immunoprecipitation. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. No cross-reactivity to other integrin b subunits.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • ITGB4
      • CD104
      • GP150
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16144 # Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
      SIZE: 1822 amino acids; 202151 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
      DOMAIN: SwissProt: P16144 The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
      DISEASE: SwissProt: P16144 # Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. & Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. This nonlethal form of junctional epidermolysis bullosa is characterized by life- long blistering of the skin, associated with hair and tooth abnormalities.
      SIMILARITY: Belongs to the integrin beta chain family. & Contains 1 Calx-beta domain. & Contains 4 fibronectin type-III domains. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe undiluted antibody solution is stable for approximately 6 months when stored -20°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Integrin β4 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin β4 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-INTEGRIN B4 SUBUNIT POLYCLONAL ANTIBODY 2978933
      RABBIT ANTI-INTEGRIN B4 SUBUNIT POLYCLONAL ANTIBODY 2952562
      RABBIT ANTI-INTEGRIN b4 SUBUNIT POLYCLONAL ANTIBODY - 2297156 2297156
      RABBIT ANTI-INTEGRIN β4 SUBUNIT - 3193870 3193870
      RABBIT ANTI-INTEGRIN β4 SUBUNIT - 3443361 3443361
      RABBIT ANTI-INTEGRIN β4 SUBUNIT - 3530632 3530632
      RABBIT ANTI-INTEGRIN β4 SUBUNIT - 3865698 3865698
      RABBIT ANTI-INTEGRIN β4 SUBUNIT POLYCLONAL ANTIBODY 3082828
      RABBIT ANTI-INTEGRIN β4 SUBUNIT POLYCLONAL ANTIBODY -2585902 2585902

      Related Products & Applications

      Related Products

      Catalog Number Description  
      MAB2058 Anti-Integrin β4 Antibody, clone ASC-3 Show Pricing & Availability
      MAB2058Z Anti-Integrin β4 Antibody, clone ASC-3, azide free Show Pricing & Availability
      MAB2059 Anti-Integrin β4 Antibody, clone ASC-8 Show Pricing & Availability
      MAB2059-C Anti-Integrin B4 Antibody, clone ASC-8, Azide & Ascites Free Show Pricing & Availability
      MAB2059Z Anti-Integrin β4 Antibody, clone ASC-8 (Azide Free) Show Pricing & Availability
      MAB2060 Anti-Integrin β4 Antibody, clone ASC-9 Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies