CP11L Anti-Integrin α₃ Mouse mAb (P1B5)

This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.

Anti-Integrin α₃ Mouse mAb (P1B5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP11L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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CP11L-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the α3-subunit of the α3/β1 integrin complex protein in human mononuclear cells.
      Catalogue NumberCP11L
      Brand Family Calbiochem®
      References
      ReferencesAlbelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
      Cheresh, D.A. et al. 1990. Cell 57, 59.
      Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
      Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
      Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
      Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
      Hynes, R.O. 1987 Cell 48, 549.
      Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlA2058 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873. Cell Inhibition Assays Carter, W.G. et al. 1990. J. Cell Biol. 110, 1387.
      Key Applications Immunoprecipitation
      Not Western Blot
      Not Paraffin Sections
      Cell Inhibition Assays
      Flow Cytometry
      Immunofluorescence
      Application NotesCell Inhibition Assays (1-5 µg/ml, see application references)
      Flow Cytometry (1-5 µg/ml)
      Immunoblotting (not recommended)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (not recommended)
      Application CommentsWill partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenhuman mononuclear cells
      ImmunogenHuman
      CloneP1B5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Integrin α₃ Mouse mAb (P1B5) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin α₃ Mouse mAb (P1B5) Certificates of Analysis

      TitleLot Number
      CP11L

      References

      Reference overview
      Albelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
      Cheresh, D.A. et al. 1990. Cell 57, 59.
      Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
      Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
      Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
      Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
      Hynes, R.O. 1987 Cell 48, 549.
      Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-March-2008 JSW
      ApplicationCell Inhibition Assays (1-5 µg/ml, see application references)
      Flow Cytometry (1-5 µg/ml)
      Immunoblotting (not recommended)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.
      BackgroundThe integrins are a family of cell surface glycoproteins that mediate both cell-cell adhesion and cell-substratum interactions. They are heterodimers consisting of noncovalently associated and subunits. Recently, it has been shown that there are six distinct subunits, and that some subunits may be associated with more than one βchain. The β1 or VLA family of integrins has the largest number of members and share the common β1 subunit. Studies have indicated that different integrins function in various combinations to mediate cell adhesion in different tissues. The α3/β1 integrin, also termed VLA3 or CD49c, has been shown to interact primarily with secreted, laminin-containing extracellular matrix.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman mononuclear cells
      CloneP1B5
      IsotypeIgG₁
      Specieshuman
      Positive controlA2058 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsWill partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
      Toxicity Standard Handling
      ReferencesAlbelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
      Cheresh, D.A. et al. 1990. Cell 57, 59.
      Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
      Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
      Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
      Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
      Hynes, R.O. 1987 Cell 48, 549.
      Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
      Application referencesOriginal Clone Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873. Cell Inhibition Assays Carter, W.G. et al. 1990. J. Cell Biol. 110, 1387.