CP11L Anti-Integrin α₃ Mouse mAb (P1B5)

This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.

Anti-Integrin α₃ Mouse mAb (P1B5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP11L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      CP11L-100UG
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      Limited AvailabilityLimited Availability
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      Discontinued
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      Available
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          		 Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the α3-subunit of the α3/β1 integrin complex protein in human mononuclear cells.
          Catalogue NumberCP11L
          Brand Family Calbiochem®
          References
          ReferencesAlbelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
          Cheresh, D.A. et al. 1990. Cell 57, 59.
          Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
          Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
          Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
          Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
          Hynes, R.O. 1987 Cell 48, 549.
          Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
          Product Information
          FormLyophilized
          FormulationLyophilized from a volatile buffer, 100 µg BSA.
          Positive controlA2058 cells
          PreservativeNone
          Applications
          Application ReferencesOriginal Clone Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873. Cell Inhibition Assays Carter, W.G. et al. 1990. J. Cell Biol. 110, 1387.
          Key Applications Immunoprecipitation
          Not Western Blot
          Not Paraffin Sections
          Cell Inhibition Assays
          Flow Cytometry
          Immunofluorescence
          Application NotesCell Inhibition Assays (1-5 µg/ml, see application references)
          Flow Cytometry (1-5 µg/ml)
          Immunoblotting (not recommended)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Paraffin Sections (not recommended)
          Application CommentsWill partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenhuman mononuclear cells
          ImmunogenHuman
          CloneP1B5
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Integrin α₃ Mouse mAb (P1B5) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Integrin α₃ Mouse mAb (P1B5) Certificates of Analysis

          TitleLot Number
          CP11L

          References

          Reference overview
          Albelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
          Cheresh, D.A. et al. 1990. Cell 57, 59.
          Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
          Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
          Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
          Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
          Hynes, R.O. 1987 Cell 48, 549.
          Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-March-2008 JSW
          ApplicationCell Inhibition Assays (1-5 µg/ml, see application references)
          Flow Cytometry (1-5 µg/ml)
          Immunoblotting (not recommended)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Paraffin Sections (not recommended)
          DescriptionPurified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.
          BackgroundThe integrins are a family of cell surface glycoproteins that mediate both cell-cell adhesion and cell-substratum interactions. They are heterodimers consisting of noncovalently associated and subunits. Recently, it has been shown that there are six distinct subunits, and that some subunits may be associated with more than one βchain. The β1 or VLA family of integrins has the largest number of members and share the common β1 subunit. Studies have indicated that different integrins function in various combinations to mediate cell adhesion in different tissues. The α3/β1 integrin, also termed VLA3 or CD49c, has been shown to interact primarily with secreted, laminin-containing extracellular matrix.
          HostMouse
          Immunogen speciesHuman
          Immunogenhuman mononuclear cells
          CloneP1B5
          IsotypeIgG₁
          Specieshuman
          Positive controlA2058 cells
          FormLyophilized
          FormulationLyophilized from a volatile buffer, 100 µg BSA.
          PreservativeNone
          CommentsWill partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
          Toxicity Standard Handling
          ReferencesAlbelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
          Cheresh, D.A. et al. 1990. Cell 57, 59.
          Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
          Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
          Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
          Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
          Hynes, R.O. 1987 Cell 48, 549.
          Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
          Application referencesOriginal Clone Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873. Cell Inhibition Assays Carter, W.G. et al. 1990. J. Cell Biol. 110, 1387.