Will partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
human mononuclear cells
Immunogen
Human
Clone
P1B5
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
Anti-Integrin α₃ Mouse mAb (P1B5) Certificates of Analysis
Title
Lot Number
CP11L
References
Reference overview
Albelda, S.M. and Buck, C.A. 1990. FASEB J.4, 2868. Cheresh, D.A. et al. 1990. Cell57, 59. Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823. Vogel, B, et al. 1990. J. Biol. Chem.265, 5934. Wayner, E.A. et al.1988 J. Cell Biol.107, 1881. Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179. Hynes, R.O. 1987 Cell48, 549. Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Purified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.
Background
The integrins are a family of cell surface glycoproteins that mediate both cell-cell adhesion and cell-substratum interactions. They are heterodimers consisting of noncovalently associated and subunits. Recently, it has been shown that there are six distinct subunits, and that some subunits may be associated with more than one βchain. The β1 or VLA family of integrins has the largest number of members and share the common β1 subunit. Studies have indicated that different integrins function in various combinations to mediate cell adhesion in different tissues. The α3/β1 integrin, also termed VLA3 or CD49c, has been shown to interact primarily with secreted, laminin-containing extracellular matrix.
Host
Mouse
Immunogen species
Human
Immunogen
human mononuclear cells
Clone
P1B5
Isotype
IgG₁
Species
human
Positive control
A2058 cells
Form
Lyophilized
Formulation
Lyophilized from a volatile buffer, 100 µg BSA.
Preservative
None
Comments
Will partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
Toxicity
Standard Handling
References
Albelda, S.M. and Buck, C.A. 1990. FASEB J.4, 2868. Cheresh, D.A. et al. 1990. Cell57, 59. Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823. Vogel, B, et al. 1990. J. Biol. Chem.265, 5934. Wayner, E.A. et al.1988 J. Cell Biol.107, 1881. Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179. Hynes, R.O. 1987 Cell48, 549. Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.
Application references
Original Clone
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.
Cell Inhibition Assays
Carter, W.G. et al. 1990. J. Cell Biol.110, 1387.