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407140 Anti-IP₃ Receptor Mouse mAb (IPR.1)

407140
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
B, Ca, H, M, Porcine, Rb, R M Monoclonal Antibody

Pricing & Availability

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407140-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecognizes all of the ~260 kDa IP3 receptor subtypes.
      Catalogue Number407140
      Brand Family Calbiochem®
      References
      ReferencesBourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
      Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
      Positive controlLymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Cavallini, L., et al. 1996. J. Biol. Chem. 271, 5545. Immunoprecipitation Jho, D., et al. 2005. Circ. Res. 96, 1282. Immunohistochemistry Lagostena, L., et al. 2001. J. Physiol. 531, 693.
      Key Applications Immunofluorescence
      Immunohistochemistry
      Immunoprecipitation
      Radioimmunoassay
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Application NotesELISA (1:1000)
      Flow Cytometry (1:500)
      Immunofluorescence (1:100)
      Immunohistochemistry (1:100; see application references)
      Immunoprecipitation (1:100; see application references)
      Radioimmunoassay (1:5000)
      Immunoblotting (see application references)
      Application CommentsSpecific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor
      CloneIPR.1
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Porcine
      • Rabbit
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-IP₃ Receptor Mouse mAb (IPR.1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-IP₃ Receptor Mouse mAb (IPR.1) Certificates of Analysis

      TitleLot Number
      407140

      References

      Reference overview
      Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
      Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-October-2008 JSW
      ApplicationELISA (1:1000)
      Flow Cytometry (1:500)
      Immunofluorescence (1:100)
      Immunohistochemistry (1:100; see application references)
      Immunoprecipitation (1:100; see application references)
      Radioimmunoassay (1:5000)
      Immunoblotting (see application references)
      DescriptionPurified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP3 receptor subtypes.
      BackgroundThe IP3 receptor is a homotetramer comprised of four ~260 kDa subunits. The IP3 receptor antibody induces significant amounts of internal Ca2+ release similar to IP3-mediated Ca2+ stimulation.
      HostMouse
      Immunogena synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor
      CloneIPR.1
      IsotypeIgG2b
      Speciesbovine, canine, human, mouse, porcine, rabbit, rat
      Positive controlLymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsSpecific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
      Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
      Application referencesImmunoblotting Cavallini, L., et al. 1996. J. Biol. Chem. 271, 5545. Immunoprecipitation Jho, D., et al. 2005. Circ. Res. 96, 1282. Immunohistochemistry Lagostena, L., et al. 2001. J. Physiol. 531, 693.