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GR11L Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
GR11L-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
Catalogue NumberGR11L
Brand Family Calbiochem®
SynonymsAnti-Insulin-Like Growth Factor Receptor, Anti-IGF-IR
References
ReferencesRoth, R. 1988. Science 239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
Rosen, O.M. 1987. Science 257, 1452.
Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
Ullrich, A., et al. 1986. EMBO J. 5, 2503.
Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
Product Information
FormLyophilized
FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
Negative controlHS27 cells
Positive controlHepG2 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesNeutralization Studies Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561. Original Clone Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
Key Applications Immunofluorescence
Immunoprecipitation
Not Western Blot
Not Paraffin Sections
Neutralization Studies
Application NotesImmunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/sample)
Neutralization Studies (1 µg/ml; see application references)
Paraffin Sections (not recommended)
Immunoblotting (not recommended)
Application CommentsBlocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpartially purified, human placenta IGF-I receptor
ImmunogenHuman
CloneαIR3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
GR11L-100UG 07790788053758

Documentation

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) SDS

Title

Safety Data Sheet (SDS) 

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) Certificates of Analysis

TitleLot Number
GR11L

References

Reference overview
Roth, R. 1988. Science 239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
Rosen, O.M. 1987. Science 257, 1452.
Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
Ullrich, A., et al. 1986. EMBO J. 5, 2503.
Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

Brochure

Title
Insulin Pathway Poster PDF ( 618 KB )

Citations

Title
  • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
  • Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.
  • Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision24-August-2007 RFH
    SynonymsAnti-Insulin-Like Growth Factor Receptor, Anti-IGF-IR
    ApplicationImmunofluorescence (1-5 µg/ml)
    Immunoprecipitation (1 µg/sample)
    Neutralization Studies (1 µg/ml; see application references)
    Paraffin Sections (not recommended)
    Immunoblotting (not recommended)
    DescriptionPurified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
    BackgroundThe insulin-like growth factors (IGFs) or somatomedins are mitogenic peptides present in the plasma of human, rat, and other species. Two distinct but chemically related IGFs have been purified from human plasma: IGF-I (Somatomedin C) and IGF-II. They are single chain polypeptides with molecular weights of 7,640 and 7,471, respectively. Types I and II IGF receptors differ in their structure and in their peptide binding specificity. Type I IGF receptors are structurally homologous to insulin receptors and contain a-subunits (MW = 130,000) that bind peptide, and b-subunits (MW = 90,000), which have intrinsic tyrosine kinase activity. The protein-tyrosine kinase activity of the insulin receptor and, presumably the IGF-I receptor, is required for insulin to stimulate all of its biological responses in cells. Type II receptors are a single polypeptide (MW = 260,000), lack protein-tyrosine kinase activity, and have been shown to be identical to the mannose-6-phosphate receptor.
    HostMouse
    Immunogen speciesHuman
    Immunogenpartially purified, human placenta IGF-I receptor
    CloneαIR3
    IsotypeIgG₁
    Specieshuman
    Positive controlHepG2 cells
    Negative controlHS27 cells
    FormLyophilized
    FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
    PreservativeNone
    CommentsBlocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
    Toxicity Standard Handling
    ReferencesRoth, R. 1988. Science 239, 1269.
    Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
    Rosen, O.M. 1987. Science 257, 1452.
    Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
    Ullrich, A., et al. 1986. EMBO J. 5, 2503.
    Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
    Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
    Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
    Citation
  • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
  • Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.
  • Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
  • Application referencesNeutralization Studies Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561. Original Clone Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.