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AB5702 Anti-HES-1 Antibody

AB5702
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5702
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-HES-1 Antibody
      Alternate Names
      • Hairy 1
      Background InformationHes1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and Hes1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human neural stem cells, Raji cell lysate.
      PresentationPurified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-HES-1 Antibody is validated for use in IH, WB for the detection of HES-1.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blot:
      1:200-1:1,000 dilution using ECL.

      Immunohistochemistry:
      1:200-1:1,000 dilution of a previous lot worked.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide.
      HostRabbit
      SpecificityRecognizes HES-1 (Hairy family), a Helix-loop-helix class of transcription factor.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and rodent. Reactivity with other species has not been confirmed.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
      Gene Symbol
      • HES1
      • Hes1
      • HL
      • HHL
      • FLJ20408
      • HRY
      • HES-1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14469 # Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity).
      SIZE: 280 amino acids; 29541 Da
      SUBUNIT: Transcription repression requires formation of a complex with a corepressor protein (Groucho) such as TLE2 (By similarity). Interacts with SIRT1.
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: Q14469 Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). & The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.
      SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 Orange domain. ... hide » see more »
      Molecular Weight29 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications