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MAB302 Anti-Glutamine Synthetase Antibody, clone GS-6

MAB302
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, ShELISA, IHC, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB302
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutamine Synthetase Antibody, clone GS-6
      Background InformationGlutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain tissue, rat brain cytosolic fraction extract
      PresentationPurified immunoglobulin. Liquid in buffer containing 50% glycerol, BSA and 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Glutamine Synthetase using this Anti-Glutamine Synthetase Antibody, clone GS-6 validated for use in ELISA, IH, IH(P) & WB with more than 45 product citations.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern blot: 1:1,000-1:5,000

      Immunohistochemistry on rat brain

      ELISA

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenGlutamine synthetase purified from sheep brain.
      CloneGS-6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts strongly with a single band on Western blots of sheep brain and rat brain. Glutamine synthetase, an enzyme that catalyzes the amination of glutamic acid to form glutamine, is found in mammals as an octamer of eight identical 45 kDa subunits. Glutamine synthetase activity has been shown to be a useful marker of astrocytes and an important differentiation feature in retina. Glutamine synthetase is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]
      Gene Symbol
      • GLUL
      • GLNS
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15104 # Catalyzes the conversion of ammonia and L-glutamate to L-glutamine.
      SIZE: 373 amino acids; 42064 Da
      SUBUNIT: Homooctamer.
      SUBCELLULAR LOCATION: Cytoplasm.
      DISEASE:SwissProt: P15104 # Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
      SIMILARITY: SwissProt: P15104 ## Belongs to the glutamine synthetase family.
      Molecular Weight45 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Glutamine Synthetase Antibody, clone GS-6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glutamine Synthetase Antibody, clone GS-6 Certificates of Analysis

      TitleLot Number
      Anti-Glutamine Synthetase, clone GS-6 3074993
      Anti-Glutamine Synthetase, clone GS-6 - 2135082 2135082
      Anti-Glutamine Synthetase, clone GS-6 - 2383959 2383959
      Anti-Glutamine Synthetase, clone GS-6 - 2426453 2426453
      Anti-Glutamine Synthetase, -2519258 2519258
      Anti-Glutamine Synthetase, -2571582 2571582
      Anti-Glutamine Synthetase, -2604816 2604816
      Anti-Glutamine Synthetase, -2651992 2651992
      Anti-Glutamine Synthetase, -2676275 2676275
      Anti-Glutamine Synthetase, -2739399 2739399

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      MAB302-KC Anti-Glutamine Synthetase,clone GS-6(1mg) KC Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies