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MAB2263 Anti-GluR1-NT (NT) Antibody, clone RH95

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MAB2263
100 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IP, ICC, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2263
DescriptionAnti-GluR1-NT (NT) Antibody, clone RH95
Alternate Names
  • AMPA 1
  • AMPA-selective glutamate receptor 1
  • Glutamate receptor ionotropic, AMPA 1
  • glutamate receptor, ionotropic, AMPA 1
Background InformationGlutamate receptors (GluRs) are a diverse group responsible for mediating most of the excitatory synaptic transmission in the CNS of vertebrates. They can be categorized as ionotropic or metabotropic and subcategorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this position is occupied by a Gln residue. The insertion or removal of GluR1/GluR4 oligomeric channels from postsynaptic membranes appears to be LTP/LTD activity dependent while GluR2/GluR3 oligomers are continuously cycling.
References
Product Information
FormatPurified
Control
  • Rat brain lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GluR1-NT (N-terminus) Antibody, clone RH95 is an antibody against GluR1-NT (N-terminus) for use in WB, IP, IC, ELISA.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
  • ELISA
Biological Information
ImmunogenThyroglobulin-conjugated linear peptide of rat GluR1 designed around the N-terminus.
EpitopeN-terminus
CloneRH95
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes GluR1 at the N-terminus.
IsotypeIgG2aκ
Species Reactivity
  • Rat
Species Reactivity NoteProven to react with rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryMouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning. [RGD]
Gene Symbol
  • GLUH1
  • GLUR1
  • GLURA
  • GluR-1
  • GluR-A
  • GluR-K1
  • HBGR1
  • MGC133252
  • GRIA1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

SUBUNIT STRUCTURE: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2, HIP1 and probably RASGRF2.

SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note: Interaction with CACNG2 promotes cell surface expression.

TISSUE SPECIFICITY: Detected in cerebellum (at protein level).

PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis By similarity.

POLYMORPHISM: Both variants Ser-710 and Thr-710 are phosphorylated at this position.

MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

SEQUENCE SIMILARITIES: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification]
Molecular Weight 110 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in rat brain lysate.

Western Blot Analysis: A 1:1000 dilutions of this antibody detected GluR1 in 10 µg of rat brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB2263 04053252617799

Documentation

Anti-GluR1-NT (NT) Antibody, clone RH95 SDS

Title

Safety Data Sheet (SDS) 

Anti-GluR1-NT (NT) Antibody, clone RH95 Certificates of Analysis

TitleLot Number
Anti-GluR1-NT (N-terminus), -2700992 2700992
Anti-GluR1-NT (N-terminus), -2780485 2780485
Anti-GluR1-NT (N-terminus), -2814785 2814785
Anti-GluR1-NT (N-terminus), clone RH95 - 2384014 2384014
Anti-GluR1-NT (N-terminus), clone RH95 - 2428724 2428724
Anti-GluR1-NT (N-terminus), clone RH95 - 1967284 1967284
Anti-GluR1-NT (N-terminus), clone RH95 - 2000838 2000838
Anti-GluR1-NT (N-terminus), clone RH95 - 2035081 2035081
Anti-GluR1-NT (N-terminus), clone RH95 - 2073645 2073645
Anti-GluR1-NT (N-terminus), clone RH95 - 2154116 2154116

References

Reference overviewApplicationSpeciesPub Med ID
The intellectual disability protein RAB39B selectively regulates GluA2 trafficking to determine synaptic AMPAR composition.
Mignogna, ML; Giannandrea, M; Gurgone, A; Fanelli, F; Raimondi, F; Mapelli, L; Bassani, S; Fang, H; Van Anken, E; Alessio, M; Passafaro, M; Gatti, S; Esteban, JA; Huganir, R; D'Adamo, P
Nature communications  6  6504  2015

Show Abstract
Western Blotting25784538 25784538
STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs.
Garcia-Alvarez, G; Lu, B; Yap, KA; Wong, LC; Thevathasan, JV; Lim, L; Ji, F; Tan, KW; Mancuso, JJ; Tang, W; Poon, SY; Augustine, GJ; Fivaz, M
Molecular biology of the cell  26  1141-59  2015

Show Abstract
25609091 25609091
Loss of F-box only protein 2 (Fbxo2) disrupts levels and localization of select NMDA receptor subunits, and promotes aberrant synaptic connectivity.
Atkin, G; Moore, S; Lu, Y; Nelson, RF; Tipper, N; Rajpal, G; Hunt, J; Tennant, W; Hell, JW; Murphy, GG; Paulson, H
The Journal of neuroscience : the official journal of the Society for Neuroscience  35  6165-78  2015

Show Abstract
25878288 25878288
PKMζ knockdown disrupts post-ischemic long-term potentiation via inhibiting postsynaptic expression of aminomethyl phosphonic acid receptors.
Cheng, N; Hu, X; Tian, T; Lu, W
Journal of biomedical research  29  241-9  2015

Show Abstract
26060448 26060448
Impaired spatial memory and enhanced long-term potentiation in mice with forebrain-specific ablation of the Stim genes.
Garcia-Alvarez, G; Shetty, MS; Lu, B; Yap, KA; Oh-Hora, M; Sajikumar, S; Bichler, Z; Fivaz, M
Frontiers in behavioral neuroscience  9  180  2015

Show Abstract
26236206 26236206
Increased response to glutamate in small diameter dorsal root ganglion neurons after sciatic nerve injury.
Gong, K; Kung, LH; Magni, G; Bhargava, A; Jasmin, L
PloS one  9  e95491  2014

Show Abstract
Immunofluorescence24748330 24748330
Mint proteins are required for synaptic activity-dependent amyloid precursor protein (APP) trafficking and amyloid β generation.
Sullivan SE, Dillon GM, Sullivan JM, Ho A.
J Biol Chem  289(22)  15374-83  2014

Show Abstract
Immunoblotting (Western)24742670 24742670
Activity-dependent FUS dysregulation disrupts synaptic homeostasis.
Sephton, CF; Tang, AA; Kulkarni, A; West, J; Brooks, M; Stubblefield, JJ; Liu, Y; Zhang, MQ; Green, CB; Huber, KM; Huang, EJ; Herz, J; Yu, G
Proceedings of the National Academy of Sciences of the United States of America  111  E4769-78  2014

Show Abstract
25324524 25324524
The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation.
Nakano-Kobayashi, A; Tai, Y; Nadif Kasri, N; Van Aelst, L
The Journal of neuroscience : the official journal of the Society for Neuroscience  34  8665-71  2014

Show Abstract
24966368 24966368
Kainate receptor post-translational modifications differentially regulate association with 4.1N to control activity-dependent receptor endocytosis.
Copits, BA; Swanson, GT
The Journal of biological chemistry  288  8952-65  2013

Show Abstract
Immunofluorescence23400781 23400781

Brochure

Title
Pathways and Biomarkers of Glutamatergic Synapse Flyer