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AB9598 Anti-Glial fibrillary acidic protein δ Antibody

AB9598
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB9598
      Replaces04-1031; 04-1062
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glial fibrillary acidic protein δ Antibody
      Alternate Names
      • GFAPdelta
      Background InformationHuman Glial Fibrillary Acidic Protein delta (GFAP-delta) is a GFAP protein isoform that is encoded by an alternative splice variant of the GFAP-gene. As a result, GFAP-delta protein differs from the predominant splice form, GFAP-alpha, by its C-terminal protein sequence. GFAP-delta protein is not expressed by all GFAP expressing astrocytes but specifically by a subpopulation located in the subpial zone of the cerebral cortex, the subgranular zone of the hippocampus and, most intensely, by a ribbon of astrocytes following the ependymal layer of the cerebral ventricles. Therefore, at least in the sub ventricular zone (SVZ), GFAP-delta specifically marks the population of astrocytes that contain the neural stem cells in the adult human brain. Interestingly, the SVZ astrocytes actively splice GFAP-delta transcripts, in contrast to astrocytes adjacent to this layer. Data shows that GFAP-delta protein, unlike GFAP-alpha, is not upregulated in astrogliosis. Data indicates a different functional role for GFAP-delta in astrocyte physiology. Transfection studies showed that GFAP-delta protein expression has a negative effect on GFAP filament formation, and therefore could be important for modulating intermediate filament cytoskeletal properties, possibly facilitating astrocyte motility. Further studies on GFAP-delta and the cells that express it are important for gaining insights into its function during differentiation, migration and during health and disease (Roelofs, RF, et al., Glia (2005) 52:289-300.).
      References
      Product Information
      FormatSerum
      PresentationRabbit serum. Liquid.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Glial fibrillary acidic protein δ Antibody is an antibody against Glial fibrillary acidic protein δ for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1:500 with overnight incubation. The antibody recognizes the ~60 kDa GFAP-delta protein.

      Immunohistochemistry: 1:500 incubated for 36-48 hours at 2-8°C on paraffin embedded tissue sections. It is suggested that the tissue be treated with microwave antigen retrieval prior to staining.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from human GFAP-delta.
      HostRabbit
      SpecificityGlial Fibrillary Acidic Protein-delta (GFAP-delta). The antibody does not recognize GFAP-alpha protein.
      Species Reactivity
      • Human
      Species Reactivity NoteDoes NOT react with mouse or rat GFAP-delta.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
      Gene Symbol
      • GFAP
      • FLJ45472
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SIZE: 432 amino acids; 49880 Da
      SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
      DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
      SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Glial fibrillary acidic protein δ Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glial fibrillary acidic protein δ Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN- Delta (GFAP-Delta)_2849056 2849056
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-d (GFAP-d) POLYCLONAL ANTIBODY - 2054780 2054780
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-d (GFAP-d) POLYCLONAL ANTIBODY - 2211010 2211010
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-δ (GFAP-δ) POLYCLONAL ANTIBODY - 2462759 2462759
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-δ (GFAP-δ) - 3220348 3220348
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-δ (GFAP-δ) - 3256025 3256025
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-δ (GFAP-δ) - 3878382 3878382
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-δ (GFAP-δ) -2860220 2860220
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-δ (GFAP-δ) POLYCLONAL ANTIBODY 3043072
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-δ (GFAP-δ) POLYCLONAL ANTIBODY 2968296

      References

      Reference overviewApplicationPub Med ID
      Neuronal inclusions of α-synuclein contribute to the pathogenesis of Krabbe disease.
      Smith, BR; Santos, MB; Marshall, MS; Cantuti-Castelvetri, L; Lopez-Rosas, A; Li, G; van Breemen, R; Claycomb, KI; Gallea, JI; Celej, MS; Crocker, SJ; Givogri, MI; Bongarzone, ER
      The Journal of pathology  232  509-21  2014

      Show Abstract
      24415155 24415155
      Subventricular zone neural progenitors from rapid brain autopsies of elderly subjects with and without neurodegenerative disease.
      Leonard, BW; Mastroeni, D; Grover, A; Liu, Q; Yang, K; Gao, M; Wu, J; Pootrakul, D; van den Berge, SA; Hol, EM; Rogers, J
      The Journal of comparative neurology  515  269-94  2009

      Show Abstract Full Text Article
      19425077 19425077
      Olig2-induced neural stem cell differentiation involves downregulation of Wnt signaling and induction of Dickkopf-1 expression.
      Ahn, SM; Byun, K; Kim, D; Lee, K; Yoo, JS; Kim, SU; Jho, EH; Simpson, RJ; Lee, B
      PloS one  3  e3917  2008

      Show Abstract
      Immunocytochemistry19093005 19093005

      Data Sheet

      Title
      RABBIT ANTI-GLIAL FIBRILLARY ACIDIC PROTEIN-δ (GFAP-δ)

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies