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MAB3402 Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

MAB3402
40 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Po, Ch, B, RbICC, IHC, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3402
      Replaces04-1031; 04-1062
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5
      Alternate Names
      • GFAP
      Background InformationGlial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain tissue, Astrocyte culture.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry:
      5 μg/mL of a previous lot was used.

      Immunohistochemistry:
      5 µg/mL

      Immunoblotting:
      Recognizes a 51kDa protein in reducing westerns of total brain lysates.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified glial filament (Debus, E., 1983).
      CloneGA5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Pig
      • Chicken
      • Bovine
      • Rabbit
      Species Reactivity NoteHuman, mouse, and rat. Expected to cross-react with porcine, chicken, bovine, and rabbit.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
      Gene Symbol
      • GFAP
      • FLJ45472
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SIZE: 432 amino acids; 49880 Da
      SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
      DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
      SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse brain lysate.

      Western Blot Analysis:
      1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
      DO NOT FREEZE.
      Packaging Information
      Material Size40 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 Certificates of Analysis

      TitleLot Number
      Anti-Glial Fibrillary Acidic Protein, -2739319 2739319
      Anti-Glial Fibrillary Acidic Protein, -2780472 2780472
      Anti-Glial Fibrillary Acidic Protein, -2814862 2814862
      Anti-Glial Fibrillary Acidic Protein, clone GA5 2465212
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2118035 2118035
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2430439 2430439
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 1944055 1944055
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 1993774 1993774
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2019952 2019952
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2049225 2049225

      References

      Reference overviewApplicationSpeciesPub Med ID
      Localization of reelin signaling pathway components in murine midbrain and striatum.
      Sharaf, A; Rahhal, B; Spittau, B; Roussa, E
      Cell and tissue research  359  393-407  2015

      Show Abstract
      Mouse25418135 25418135
      Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity.
      Ko, AR; Hyun, HW; Min, SJ; Kim, JE; Kang, TC
      Molecular brain  8  58  2015

      Show Abstract
      26438559 26438559
      Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory.
      Orr, AG; Hsiao, EC; Wang, MM; Ho, K; Kim, DH; Wang, X; Guo, W; Kang, J; Yu, GQ; Adame, A; Devidze, N; Dubal, DB; Masliah, E; Conklin, BR; Mucke, L
      Nature neuroscience  18  423-34  2015

      Show Abstract
      25622143 25622143
      Transiently lowering tumor necrosis factor-α synthesis ameliorates neuronal cell loss and cognitive impairments induced by minimal traumatic brain injury in mice.
      Baratz, R; Tweedie, D; Wang, JY; Rubovitch, V; Luo, W; Hoffer, BJ; Greig, NH; Pick, CG
      Journal of neuroinflammation  12  45  2015

      Show Abstract
      25879458 25879458
      Involvement of phosphatase and tensin homolog deleted from chromosome 10 in rodent model of neuropathic pain.
      Huang, SY; Sung, CS; Chen, WF; Chen, CH; Feng, CW; Yang, SN; Hung, HC; Chen, NF; Lin, PR; Chen, SC; Wang, HM; Chu, TH; Tai, MH; Wen, ZH
      Journal of neuroinflammation  12  59  2015

      Show Abstract
      25889774 25889774
      Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal β-amyloid deposition in the human brain.
      Xue, ZQ; He, ZW; Yu, JJ; Cai, Y; Qiu, WY; Pan, A; Gai, WP; Cai, H; Luo, XG; Ma, C; Yan, XX
      BMC neurology  15  71  2015

      Show Abstract
      25934480 25934480
      Netrin-5 is highly expressed in neurogenic regions of the adult brain.
      Yamagishi, S; Yamada, K; Sawada, M; Nakano, S; Mori, N; Sawamoto, K; Sato, K
      Frontiers in cellular neuroscience  9  146  2015

      Show Abstract
      25941474 25941474
      Human Embryonic Stem Cell-Derived Progenitors Assist Functional Sensory Axon Regeneration after Dorsal Root Avulsion Injury.
      Hoeber, J; Trolle, C; Konig, N; Du, Z; Gallo, A; Hermans, E; Aldskogius, H; Shortland, P; Zhang, SC; Deumens, R; Kozlova, EN
      Scientific reports  5  10666  2015

      Show Abstract
      Immunohistochemistry26053681 26053681
      Hepatic stellate cells contribute to progenitor cells and liver regeneration.
      Kordes, C; Sawitza, I; Götze, S; Herebian, D; Häussinger, D
      The Journal of clinical investigation  124  5503-15  2014

      Show Abstract
      25401473 25401473
      Neurochemical properties of BDNF-containing neurons projecting to rostral ventromedial medulla in the ventrolateral periaqueductal gray.
      Yin, JB; Wu, HH; Dong, YL; Zhang, T; Wang, J; Zhang, Y; Wei, YY; Lu, YC; Wu, SX; Wang, W; Li, YQ
      Frontiers in neural circuits  8  137  2014

      Show Abstract
      25477786 25477786

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      Alternative Format

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies