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AB5541 Anti-Glial Fibrillary Acidic Protein Antibody

AB5541
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, PoICC, IHC, IH(P), WBChAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5541
      Replaces04-1031; 04-1062
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glial Fibrillary Acidic Protein Antibody
      Alternate Names
      • GFAP
      Background InformationGlial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Astrocytes, astrocytomas, neural stem cells.
      PresentationPurified chicken polyclonal in buffer containing PBS containing 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry(paraffin):
      Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.

      Immunocytochemistry:
      1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

      Western blot:
      1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.

      Immunohistochemistry:
      1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.

      Optimal working dilutions must be determined by the end user.

      STAINING PATTERN:

      The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
      Biological Information
      ImmunogenPurified bovine GFAP.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostChicken
      SpecificityGlial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Pig
      Species Reactivity NoteHuman, rat, and mouse. Expected to cross-react with bovine and porcine. Other species have not yet been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
      Gene Symbol
      • GFAP
      • FLJ45472
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SIZE: 432 amino acids; 49880 Da
      SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
      DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
      SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family. ...
      Molecular Weight55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Glial Fibrillary Acidic Protein Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glial Fibrillary Acidic Protein Antibody Certificates of Analysis

      TitleLot Number
      Anti-Glial Fibrillary Acidic Protein - 2118930 2118930
      Anti-Glial Fibrillary Acidic Protein - 2386488 2386488
      Anti-Glial Fibrillary Acidic Protein - 2395670 2395670
      Anti-Glial Fibrillary Acidic Protein - 2428445 2428445
      Anti-Glial Fibrillary Acidic Protein - 2459003 2459003
      Anti-Glial Fibrillary Acidic Protein - 1973208 1973208
      Anti-Glial Fibrillary Acidic Protein - 2010648 2010648
      Anti-Glial Fibrillary Acidic Protein - 2053332 2053332
      Anti-Glial Fibrillary Acidic Protein - 2189693 2189693
      Anti-Glial Fibrillary Acidic Protein - 2297161 2297161

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies