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06-896 Anti-GFP (Green Fluorescent Protein) Antibody

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt ICC, WB Ch Purified Polyclonal Antibody
      Catalogue Number06-896
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GFP (Green Fluorescent Protein) Antibody
      Alternate Names
      • Green fluorescent protein
      Background InformationGreen fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells.
      Product Information
      • Rat brain lysate, cells expressing GFP protein.
      PresentationPurified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.
      Quality LevelMQ100
      ApplicationAnti-GFP (Green Fluorescent Protein) Antibody detects level of GFP (Green Fluorescent Protein) & has been published & validated for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.
      Biological Information
      ImmunogenHis-tagged green fluorescent protein of Aequorea victoria.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins.
      Species Reactivity
      • Vertebrates
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • GFP
      Purification MethodAmmonium Sulfate Precipitation and PEG
      UniProt Number
      UniProt SummaryFUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
      Absorption: Abs(max)=395 nm;
      Note=Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm;
      SUBUNIT: Monomer.
      TISSUE SPECIFICITY: Photocytes.
      PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
      BIOTECHNOLOGY: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
      BIOTECHNOLOGY: Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
      SIMILARITY: Belongs to the GFP family.
      Molecular Weight30 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information


      Anti-GFP (Green Fluorescent Protein) Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-GFP (Green Fluorescent Protein) Antibody Certificates of Analysis

      TitleLot Number
      Anti-GFP (Green Fluorescent Protein) - 33137523313752
      Anti-GFP (Green Fluorescent Protein) (chicken polyclonal IgY) - DAM1430003DAM1430003
      Anti-GFP (Green Fluorescent Protein) - 23826242382624
      Anti-GFP (Green Fluorescent Protein) - 24532372453237
      Anti-GFP (Green Fluorescent Protein) - 1790117901
      Anti-GFP (Green Fluorescent Protein) - 19645431964543
      Anti-GFP (Green Fluorescent Protein) - 20287292028729
      Anti-GFP (Green Fluorescent Protein) - 2050520505
      Anti-GFP (Green Fluorescent Protein) - 21975062197506
      Anti-GFP (Green Fluorescent Protein) - 2269422694


      Reference overviewApplicationPub Med ID
      Interplay between glucose and leptin signalling determines the strength of GABAergic synapses at POMC neurons.
      Lee, DK; Jeong, JH; Chun, SK; Chua, S; Jo, YH
      Nature communications  6  6618  2015

      Show Abstract
      25808323 25808323
      Distribution of nanoparticles throughout the cerebral cortex of rodents and non-human primates: Implications for gene and drug therapy.
      Salegio, EA; Streeter, H; Dube, N; Hadaczek, P; Samaranch, L; Kells, AP; San Sebastian, W; Zhai, Y; Bringas, J; Xu, T; Forsayeth, J; Bankiewicz, KS
      Frontiers in neuroanatomy  8  9  2014

      Show Abstract
      24672434 24672434
      An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration.
      Azzarelli, R; Pacary, E; Garg, R; Garcez, P; van den Berg, D; Riou, P; Ridley, AJ; Friedel, RH; Parsons, M; Guillemot, F
      Nature communications  5  3405  2014

      Show Abstract
      24572910 24572910
      In vivo optogenetic control of striatal and thalamic neurons in non-human primates.
      Galvan, A; Hu, X; Smith, Y; Wichmann, T
      PloS one  7  e50808  2012

      Show Abstract
      23226390 23226390
      Association of shank 1A scaffolding protein with cone photoreceptor terminals in the mammalian retina.
      Stella, SL; Vila, A; Hung, AY; Rome, ME; Huynh, U; Sheng, M; Kreienkamp, HJ; Brecha, NC
      PloS one  7  e43463  2012

      Show Abstract
      22984429 22984429
      MARK2/Par-1 guides the directionality of neuroblasts migrating to the olfactory bulb.
      Sheyla Mejia-Gervacio,Kerren Murray,Tamar Sapir,Richard Belvindrah,Orly Reiner,Pierre-Marie Lledo
      Molecular and cellular neurosciences  49  2012

      Show Abstract
      22061967 22061967
      Bcl-xL regulates metabolic efficiency of neurons through interaction with the mitochondrial F1FO ATP synthase.
      Alavian, KN; Li, H; Collis, L; Bonanni, L; Zeng, L; Sacchetti, S; Lazrove, E; Nabili, P; Flaherty, B; Graham, M; Chen, Y; Messerli, SM; Mariggio, MA; Rahner, C; McNay, E; Shore, GC; Smith, PJ; Hardwick, JM; Jonas, EA
      Nature cell biology  13  1224-33  2011

      Show Abstract
      Western Blotting21926988 21926988
      NKCC1 controls GABAergic signaling and neuroblast migration in the postnatal forebrain.
      Mejia-Gervacio, S; Murray, K; Lledo, PM
      Neural development  6  4  2011

      Show Abstract
      Immunohistochemistry21284844 21284844
      Oligodendrocyte PTEN is required for myelin and axonal integrity, not remyelination.
      Harrington, EP; Zhao, C; Fancy, SP; Kaing, S; Franklin, RJ; Rowitch, DH
      Annals of neurology  68  703-16  2010

      Show Abstract
      Western Blotting20853437 20853437
      Nutrition-responsive glia control exit of neural stem cells from quiescence.
      Chell, JM; Brand, AH
      Cell  143  1161-73  2010

      Show Abstract
      21183078 21183078

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      Life Science Research > Antibodies and Assays > Primary Antibodies