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CB1001 Anti-GAPDH Mouse mAb (6C5)

CB1001
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ca, Ch, F, Fg, H, M, Porcine, Rb, R M Monoclonal Antibody

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CB1001-500UG
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      Plastic ampoule 500 μg
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      Description
      OverviewRecognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
      Catalogue NumberCB1001
      Brand Family Calbiochem®
      SynonymsAnti-Glyceraldehyde-3-Phosphate Dehydrogenase
      Application Data
      Detection of rat GAPDH by immunocytochemistry. Sample: A-10 cell line (rat aortic smooth muscle cells). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (assay dependent of Primary Antibody). Detection: fluorescence.

      Detection of GAPDH by immunoblotting. Samples: Tissue exctract from isolated human GAPDH (lane 1), human heart (lane 2), pig heart (lane 3), goat heart (lane 4), bovine heart (lane 5), dog heart (lane 6), mouse heart (lane 7), rat heart (lane 8), rabbit heart (lane 9), and duck heart (lane 10). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (1 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Positive controlRat skeletal muscle
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-10 µg/ml; see application references)
      Immunocytochemistry (see comments)
      Application CommentsUseful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified, rabbit muscle
      ImmunogenRabbit
      Clone6C5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Canine
      • Chicken
      • Fish
      • Frog
      • Human
      • Mouse
      • Porcine
      • Rabbit
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-GAPDH Mouse mAb (6C5) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GAPDH Mouse mAb (6C5) Certificates of Analysis

      TitleLot Number
      CB1001
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-November-2007 JSW
      SynonymsAnti-Glyceraldehyde-3-Phosphate Dehydrogenase
      ApplicationELISA (see comments)
      Immunoblotting (1-10 µg/ml; see application references)
      Immunocytochemistry (see comments)
      Application Data
      Detection of rat GAPDH by immunocytochemistry. Sample: A-10 cell line (rat aortic smooth muscle cells). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (assay dependent of Primary Antibody). Detection: fluorescence.

      Detection of GAPDH by immunoblotting. Samples: Tissue exctract from isolated human GAPDH (lane 1), human heart (lane 2), pig heart (lane 3), goat heart (lane 4), bovine heart (lane 5), dog heart (lane 6), mouse heart (lane 7), rat heart (lane 8), rabbit heart (lane 9), and duck heart (lane 10). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (1 µg/ml). Detection: chemiluminescence.
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
      BackgroundGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. GAPDH is comprised of four ~36 kDa subunits with a total molecular weight of ~146 kDa. Besides functioning as a glycolytic enzyme in cytoplasm, mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recently, findings have been made concerning the role of GAPDH in differentpathologies including prostate cancer progression, programmed neuronal cell death, and age-related neuronal diseases such as Alzheimer’s Disease and Huntington’s Disease. While GAPDH is constitutively expressed in almost all tissues at high levels, there are some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types.
      HostMouse
      Immunogen speciesRabbit
      Immunogenpurified, rabbit muscle
      Clone6C5
      IsotypeIgG₁
      Speciesnot bovine, canine, chicken, fish, frog, human, mouse, porcine, rabbit, rat, not yeast
      Positive controlRat skeletal muscle
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsUseful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Toxicity Highly Toxic
      Application referencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.