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05-117 Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

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05-117
500 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, RNEUT, RIAMPurifiedMonoclonal Antibody
Description
Catalogue Number05-117
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1
Alternate Names
  • Basic fibroblast growth factor
  • basic fibroblast growth factor bFGF
  • fibroblast growth factor 2
  • fibroblast growth factor 2 (basic)
  • heparin-binding growth factor 2.
Background InformationBasic fibroblast growth factor, also known as bFGF or FGF2, is a member of the fibroblast growth factor family.
In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.
It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis.
Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Fetal liver or kidney tissue.
PresentationPurified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.
Quality LevelMQ100
Applications
ApplicationAnti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 is an antibody against FGF-2/basic FGF (neutralizing) for use in NEUT, RIA.
Key Applications
  • Neutralizing
  • Radioimmunoassay
Applications Not Recommended
  • Western Blotting
Biological Information
ImmunogenPurified bovine brain basic FGF (HBGF-2). Clone bFM-1.
Epitopea.a. 5-24
ClonebFM-1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.
IsotypeIgG1κ
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Species Reactivity NoteThis antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Gene Symbol
  • FGF2
  • Prostatropin
  • BFGF
  • prostatropin
  • HBGF-2
  • FGFB
Purification MethodAmmonium sulfate precipitation and DEAE-cellulose chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
SIZE: 155 amino acids; 17254 Da
SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.
SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family.
MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. ... hide » see more »
Molecular Weight17.5 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.

Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-117 04053252269073

Documentation

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 SDS

Title

Safety Data Sheet (SDS) 

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 Certificates of Analysis

TitleLot Number
Anti-FGF-2/basic FGF (neutralizing), -2762408 2762408
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 (mouse monoclonal IgG1k) - DAM1400105 DAM1400105
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2117974 2117974
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2135089 2135089
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2397053 2397053
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 1940140 1940140
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 1980376 1980376
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 1997385 1997385
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2004527 2004527
Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2036078 2036078

References

Reference overviewApplicationPub Med ID
Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF.
Fessler, E; Borovski, T; Medema, JP
Molecular cancer  14  157  2015

Show Abstract
26282129 26282129
High molecular weight fibroblast growth factor-2 in the human heart is a potential target for prevention of cardiac remodeling.
Santiago, JJ; McNaughton, LJ; Koleini, N; Ma, X; Bestvater, B; Nickel, BE; Fandrich, RR; Wigle, JT; Freed, DH; Arora, RC; Kardami, E
PloS one  9  e97281  2014

Show Abstract
Western Blotting24827991 24827991
Stromal heparan sulfate differentiates neuroblasts to suppress neuroblastoma growth.
Knelson, EH; Gaviglio, AL; Nee, JC; Starr, MD; Nixon, AB; Marcus, SG; Blobe, GC
The Journal of clinical investigation  124  3016-31  2014

Show Abstract
24937430 24937430
Type III TGF-β receptor promotes FGF2-mediated neuronal differentiation in neuroblastoma.
Knelson, EH; Gaviglio, AL; Tewari, AK; Armstrong, MB; Mythreye, K; Blobe, GC
The Journal of clinical investigation  123  4786-98  2013

Show Abstract
24216509 24216509
EphA2-induced angiogenesis in ewing sarcoma cells works through bFGF production and is dependent on caveolin-1.
Sáinz-Jaspeado, M; Huertas-Martinez, J; Lagares-Tena, L; Martin Liberal, J; Mateo-Lozano, S; de Alava, E; de Torres, C; Mora, J; Del Muro, XG; Tirado, OM
PloS one  8  e71449  2013

Show Abstract
23951165 23951165
Preferential accumulation and export of high molecular weight FGF-2 by rat cardiac non-myocytes.
Santiago, JJ; Ma, X; McNaughton, LJ; Nickel, BE; Bestvater, BP; Yu, L; Fandrich, RR; Netticadan, T; Kardami, E
Cardiovascular research  89  139-47  2011

Show Abstract
Western Blotting20696821 20696821
A novel mechanism of sequestering fibroblast growth factor 2 by glypican in lipid rafts, allowing skeletal muscle differentiation.
Jaime Gutiérrez,Enrique Brandan
Molecular and cellular biology  30  2010

Show Abstract Full Text Article
20100867 20100867
Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangioblast stem cells.
Candela, Maria Elena, et al.
J. Cell. Physiol., 224: 144-51 (2010)  2010

Show Abstract
20232295 20232295
Fibroblast growth factor-2 is a downstream mediator of phosphatidylinositol 3-kinase-Akt signaling in 14,15-epoxyeicosatrienoic acid-induced angiogenesis.
Zhang, B; Cao, H; Rao, GN
The Journal of biological chemistry  281  905-14  2006

Show Abstract
16286479 16286479
Postischemic intraventricular administration of FGF-2 expressing adenoviral vectors improves neurologic outcome and reduces infarct volume after transient focal cerebral ischemia in rats.
Watanabe, T; Okuda, Y; Nonoguchi, N; Zhao, MZ; Kajimoto, Y; Furutama, D; Yukawa, H; Shibata, MA; Otsuki, Y; Kuroiwa, T; Miyatake, S
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  24  1205-13  2004

Show Abstract
15545913 15545913