Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

05-101 Anti-EGFR Antibody, neutralizing, clone LA1

View Products on Sigmaaldrich.com
05-101
250 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IHC, IP, NEUT, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-101
Replaces04-337; 04-338
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-EGFR Antibody, neutralizing, clone LA1
OverviewReacts with external domain of EGF receptor on all human cells. Competes with EGF and TGF-α for binding on human cells.
Background InformationEpidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type I membrane glycoprotein that is ubiquitously expressed. It is synthesized with a signal peptide (1-24), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 25-645), a transmembrane domain (aa 646-668), and a long cytoplasmic domain (669-1210). It possesses receptor tyrosine kinase activity and upon binding of ligands activates several signaling cascades. Ligand binding is known to trigger receptor homo- and/or heterodimerization, which stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of tyrosine residues in the C-terminal domain of EGFR occurs, which results in activation of several downstream signaling cascades, including the Ras-RAF-MEK-ERK, PI3 kinase-Akt, PLCg-PKC, and STAT. EGFR phosphorylation at serine 695 is reported to be only partial and occurs only if threonine 693 is phosphorylated. EGFR also undergoes mono and polyubiquitination upon EGF stimulation. However, this ubiquitination does not affect tyrosine kinase activity of the receptor or its signaling capacity. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes.
References
Product Information
FormatPurified
Control
  • Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
PresentationLyophilized protein G Purified mouse immunoglobulin. Lyophilized from 0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing no preservatives.
Quality LevelMQ100
Applications
ApplicationDetect EGFR using this Anti-EGFR Antibody, neutralizing, clone LA1 validated for use in Immuncytochemistry, Immunohistochemistry, Immunoprecipitation, Neutralization, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Neutralizing
  • Western Blotting
Application NotesImmunocytochemistry (IC) Analysis: 2µg/mL of a representative lot detected EGFR in A431 cells.
Immunohistochemistry (Paraffin) (IHC) Analysis: A 1:50 dilution of this antibody detected EGFR in Human placenta tissue sections.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated EGFR in Huh-7.5 cells. (Diao, J., et al. (2012). J. Virol. 86(20); 10935-10949).
Western Blotting Analysis: 1 mg/mL of a representative lot detected EGFR in A431 cell lysate.
Biological Information
ImmunogenHuman A431 membrane proteins.
CloneLA1
HostMouse
SpecificityRecognizes both phosphorylated and non-phosphorylated human EGF receptors.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EGFR
  • ERBB1
  • ERBB
  • mENA
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
Molecular Weight180 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated on RIPA lysates from non-stimulated human A431 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsLyophilized: 2 years at -20°C; Rehydrated: 1 month at 4°C or 6 months at -20°C
Packaging Information
Material Size250 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-101 04053252470356

Documentation

Anti-EGFR Antibody, neutralizing, clone LA1 SDS

Title

Safety Data Sheet (SDS) 

Anti-EGFR Antibody, neutralizing, clone LA1 Certificates of Analysis

TitleLot Number
Anti-EGFR, neutralizing, clone LA1 2464554
Anti-EGFR, neutralizing, clone LA1 (mouse monoclonal IgG1) - DAM1413897 DAM1413897
Anti-EGFR, neutralizing, clone LA1 - 2024628 2024628
Anti-EGFR, neutralizing, clone LA1 - 2154196 2154196
Anti-EGFR, neutralizing, clone LA1 - 2382130 2382130
Anti-EGFR, neutralizing, clone LA1 - 2446776 2446776
Anti-EGFR, neutralizing, clone LA1 - 15606 15606
Anti-EGFR, neutralizing, clone LA1 - 16617 16617
Anti-EGFR, neutralizing, clone LA1 - 17832 17832
Anti-EGFR, neutralizing, clone LA1 - 19226 19226

References

Reference overviewApplicationPub Med ID
Crucial roles of RSK in cell motility by catalysing serine phosphorylation of EphA2.
Zhou, Y; Yamada, N; Tanaka, T; Hori, T; Yokoyama, S; Hayakawa, Y; Yano, S; Fukuoka, J; Koizumi, K; Saiki, I; Sakurai, H
Nature communications  6  7679  2015

Show Abstract
26158630 26158630
Epithelial innate immune response to Acinetobacter baumannii challenge.
Feng, Z; Jia, X; Adams, MD; Ghosh, SK; Bonomo, RA; Weinberg, A
Infection and immunity  82  4458-65  2014

Show Abstract
25114113 25114113
EGFR/Src/Akt signaling modulates Sox2 expression and self-renewal of stem-like side-population cells in non-small cell lung cancer.
Singh, S; Trevino, J; Bora-Singhal, N; Coppola, D; Haura, E; Altiok, S; Chellappan, SP
Molecular cancer  11  73  2012

Show Abstract
23009336 23009336
Studies of cell signaling in a reconstructed human epidermis exposed to sensitizers: IL-8 synthesis and release depend on EGFR activation.
Frankart, Aurélie, et al.
Archives of dermatological research, (2012)  2012

Show Abstract
22271211 22271211
EGFR and EphA2 are host factors for hepatitis C virus entry and possible targets for antiviral therapy.
Lupberger, Joachim, et al.
Nature medicine, (2011)  2011

Show Abstract
21516087 21516087
Mutant p53 initiates a feedback loop that involves Egr-1/EGF receptor/ERK in prostate cancer cells.
L Sauer,D Gitenay,C Vo,V T Baron
Oncogene  29  2010

Show Abstract Full Text Article
20190820 20190820
Acrolein-activated matrix metalloproteinase 9 contributes to persistent mucin production.
Deshmukh, HS; Shaver, C; Case, LM; Dietsch, M; Wesselkamper, SC; Hardie, WD; Korfhagen, TR; Corradi, M; Nadel, JA; Borchers, MT; Leikauf, GD
American journal of respiratory cell and molecular biology  38  446-54  2008

Show Abstract
18006877 18006877
Dexamethasone and methylprednisolone do not inhibit neuritic outgrowth while inhibiting outgrowth of fibroblasts from spiral ganglion explants.
Alexis Furze,Deanna Kralick,Anand Vakharia,Korey Jaben,Reid Graves,Eelam Adil,Adrien A Eshraghi,Thomas J Balkany,Thomas R Van de Water
Acta oto-laryngologica  128  2008

Show Abstract
17851911 17851911
Delineation of molecular mechanisms of sensitivity to lapatinib in breast cancer cell lines using global gene expression profiles.
Priti S Hegde,David Rusnak,Melissa Bertiaux,Krystal Alligood,Jay Strum,Robert Gagnon,Tona M Gilmer
Molecular cancer therapeutics  6  2007

Show Abstract
17513611 17513611
Roles of an IkappaB kinase-related pathway in human cytomegalovirus-infected vascular smooth muscle cells: a molecular link in pathogen-induced proatherosclerotic conditions.
Gravel, SP; Servant, MJ
The Journal of biological chemistry  280  7477-86  2005

Show Abstract
15619605 15619605

FAQ

QuestionAnswer
How do I prepare a non-reducing sample buffer?Non-reducing sample buffer can be made and stored for months at room temperature. Here is the formulation for a 2X Non-reducing sample buffer: Water (37ml), 0.5M Tris-HCl (10ml), Glycerol (8ml), 10% SDS (16ml), 0.05% Bromophenol Blue (5ml).

Related Products & Applications

Related Products

Alternative Format

Catalog Number Description
16-246 Anti-EGFR Antibody, neutralizing, clone LA1, Alexa Fluor® 488

Included Positive Control

Catalog Number Description
12-301 Non-Stimulated A431 Cell Lysate

Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies