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MAB2029 Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27

MAB2029
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IF, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2029
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Chondroitin Sulfate Proteoglycan using this Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27 validated for use in FC, IF & IP.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesImmunofluorescence microscopy of cells in culture (Bumoi et al., 1984; Schrappe et al., 1992). Also, Legg J et al (2003) Development 130:6049-6063 {3% PFA fixed frozen sections}.

      Immunoelectron microscopy (Bumoi et al., 1984)

      Immunoprecipitation: 1μg/10E6 cells (Bumoi et al., 1984; Morgan et al., 1981)

      FACS analysis: 1-2μg/10E6 cells

      Functional block of melanoma cell spreading (Morgan et al., 1981), glioma cell proliferation3 and tumor growth in nude mice (Morgan et al., 1981; Schrappe et al., 1992).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose™ (Morgan et al., 1981).
      Clone9.2.27
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984). Reacts with human melanoma, glioma and proliferating brain endothelial cells.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
      Gene Symbol
      • CSPG4
      • MSK16
      • MCSP
      • NG2
      • MCSPG
      • MEL-CSPG
      • HMW-MAA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.


      SIZE: 2322 amino acids; 250497 Da

      SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.

      SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).

      TISSUE SPECIFICITY: Detected only in malignant melanoma cells.



      PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).


      SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.

      MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain between 2 and 8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN MONOCLONAL ANTIBODY - 2368744 2368744
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN - 3191417 3191417
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN - 3221961 3221961
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN - 3259058 3259058
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN - 3321110 3321110
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN - 3474225 3474225
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN - 3611102 3611102
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN - 3795264 3795264
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN -2521461 2521461
      MOUSE ANTI-HUMAN CHONDROITIN SULFATE PROTEOGLYCAN -2575147 2575147

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies