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MAB2035 Anti-Chondroitin 6 Sulfate Antibody, clone MK-302

MAB2035
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ca, H, M, R, Rb, ShELISA, RIA, WBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB2035
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Chondroitin 6 Sulfate Antibody, clone MK-302
      References
      Product Information
      FormatAscites
      PresentationAscites liquid; no preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Chondroitin 6 Sulfate Antibody, clone MK-302 is an antibody against Chondroitin 6 Sulfate for use in ELISA, RIA & WB.
      Key Applications
      • ELISA
      • Radioimmunoassay
      • Western Blotting
      Application NotesELISA at 1:100-1:5,000

      RIA at 1:100-1:200.

      Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenChondroitinase ABC-digested adult human aggrecan.
      CloneMK-302
      HostMouse
      SpecificityReacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Sheep
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
      Gene Symbol
      • ACAN
      • MSK16
      • SEDK
      • CSPCP
      • AGCAN
      • AGC1
      • CSPG1
      • CSPGCP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
      SIZE: 2415 amino acids; 250193 Da
      SUBUNIT: Interacts with FBLN1 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      TISSUE SPECIFICITY: Restricted to cartilages.
      DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton.
      DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.
      PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
      DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
      SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Chondroitin 6 Sulfate Antibody, clone MK-302 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Chondroitin 6 Sulfate Antibody, clone MK-302 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CHONDROITIN-6-SULFATE MONOCLONAL ANTIBODY - 2135076 2135076
      MOUSE ANTI-CHONDROITIN-6-SULFATE MONOCLONAL ANTIBODY - 2395656 2395656
      MOUSE ANTI-CHONDROITIN-6-SULFATE - 2894586 2894586
      MOUSE ANTI-CHONDROITIN-6-SULFATE - 2906478 2906478
      MOUSE ANTI-CHONDROITIN-6-SULFATE - 3439829 3439829
      MOUSE ANTI-CHONDROITIN-6-SULFATE - 3589835 3589835
      MOUSE ANTI-CHONDROITIN-6-SULFATE -2752733 2752733
      MOUSE ANTI-CHONDROITIN-6-SULFATE -2792524 2792524
      MOUSE ANTI-CHONDROITIN-6-SULFATE MONOCLONAL ANTIBODY 2947337
      MOUSE ANTI-CHONDROITIN-6-SULFATE MONOCLONAL ANTIBODY 2883517

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies