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MAB2030 Anti-Chondroitin 4 Sulfate Antibody, clone BE-123

MAB2030
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ca, H, M, R, ShELISA, RIA, WBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB2030
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Chondroitin 4 Sulfate Antibody, clone BE-123
      References
      Product Information
      FormatAscites
      PresentationAscites fluid containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.
      Key Applications
      • ELISA
      • Radioimmunoassay
      • Western Blotting
      Application NotesELISA at 1:400-1:3,200.

      RIA at 1:50-1:6,400.

      Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
      The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)

      Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
      In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
      Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenMouse proteoglycan.
      CloneBE-123
      HostMouse
      SpecificityReacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase.
      IsotypeIgG1κ
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
      Gene Symbol
      • CSPG4
      • MSK16
      • MCSP
      • NG2
      • MCSPG
      • MEL-CSPG
      • HMW-MAA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
      SIZE: 2322 amino acids; 250497 Da
      SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.
      SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).
      TISSUE SPECIFICITY: Detected only in malignant melanoma cells.
      PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).
      SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.
      MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CHONDROITIN-4-SULFATE MONOCLONAL ANTIBODY - 2138551 2138551
      MOUSE ANTI-CHONDROITIN-4-SULFATE MONOCLONAL ANTIBODY - 2420394 2420394
      MOUSE ANTI-CHONDROITIN-4-SULFATE - 3066209 3066209
      MOUSE ANTI-CHONDROITIN-4-SULFATE - 3286076 3286076
      MOUSE ANTI-CHONDROITIN-4-SULFATE - 3496544 3496544
      MOUSE ANTI-CHONDROITIN-4-SULFATE - 3684745 3684745
      MOUSE ANTI-CHONDROITIN-4-SULFATE - 3809300 3809300
      MOUSE ANTI-CHONDROITIN-4-SULFATE - 3873581 3873581
      MOUSE ANTI-CHONDROITIN-4-SULFATE -2574085 2574085
      MOUSE ANTI-CHONDROITIN-4-SULFATE -2586492 2586492

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies