Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

17-299 Anti-Cdc42 Immunoblotting Kit

View Products on Sigmaaldrich.com
17-299
1 kit  Kit capacity: 10 immunoblots per kit
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Key Applications
WB
Description
Catalogue Number17-299
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Cdc42 Immunoblotting Kit
OverviewAnti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog
References
Product Information
Components
  • Anti-cdc42, (mouse monoclonal IgG1), Catalog # 05-542
  • Immunoblot Blocking Reagent (Cat.# 20-200)
  • Goat Anti-Mouse IgG, HRP conjugate (Cat.# 12-349)
PresentationSee Certificate of Analysis for details
Quality LevelMQ100
Applications
ApplicationThis Anti-Cdc42 Immunoblotting Kit detects Cdc42 when performing western blots.
Key Applications
  • Western Blotting
Biological Information
HostMouse
Species Reactivity
  • Human
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
Gene Symbol
  • CDC42
  • G25K
  • CDC42Hs
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P60953 # Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
SIZE: 191 amino acids; 21311 Da
SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
SIMILARITY: SwissProt: P60953 ## Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from HL-60 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of shipment.
Note: Catalog # 12-349, Goat Anti-Mouse IgG, HRP-conjugate is light sensitive, handle accordingly.
Packaging Information
Material Size1 kit
Material PackageKit capacity: 10 immunoblots per kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
17-299 04053252280443

Documentation

Anti-Cdc42 Immunoblotting Kit Certificates of Analysis

TitleLot Number
Anti-cdc42 Immunoblotting Kit 2912074
Anti-cdc42 Immunoblotting Kit 3105944
Anti-cdc42 Immunoblotting Kit 2832143
Anti-cdc42 Immunoblotting Kit - 2459580 2459580
Anti-cdc42 Immunoblotting Kit - 0701050665 0701050665
Anti-cdc42 Immunoblotting Kit - 20130 20130
Anti-cdc42 Immunoblotting Kit - 2043487 2043487
Anti-cdc42 Immunoblotting Kit - 2064535 2064535
Anti-cdc42 Immunoblotting Kit - 21680 21680
Anti-cdc42 Immunoblotting Kit - 2212028 2212028

References

Reference overviewPub Med ID
Impaired NK-cell migration in WAS/XLT patients: role of Cdc42/WASp pathway in the control of chemokine-induced beta2 integrin high-affinity state.
Helena Stabile,Claudia Carlino,Cinzia Mazza,Silvia Giliani,Stefania Morrone,Lucia D Notarangelo,Luigi D Notarangelo,Angela Santoni,Angela Gismondi
Blood  115  2010

Show Abstract Full Text Article
20130240 20130240
Impaired natural and CD16-mediated NK cell cytotoxicity in patients with WAS and XLT: ability of IL-2 to correct NK cell functional defect.
Angela Gismondi, Loredana Cifaldi, Cinzia Mazza, Silvia Giliani, Silvia Parolini, Stefania Morrone, Jordan Jacobelli, Elisabetta Bandiera, Luigi Notarangelo, Angela Santoni
Blood  104  436-43  2004

Show Abstract
15001467 15001467