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06-734 Anti-β-Catenin Antibody

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200 µg  
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Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, M, Rb, BICC, IP, WBRbPurifiedPolyclonal Antibody
Catalogue Number06-734
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-β-Catenin Antibody
Alternate Names
  • catenin (cadherin-associated protein)
  • beta 1 (88kD)
  • catenin (cadherin-associated protein), beta 1 (88kDa)
  • catenin (cadherin-associated protein), beta 1, 88kDa
Background Informationβ-catenin plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell-cell adherens junction (AJ) molecules, components of its degradation complex, and transcription factors.
β-Catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. β-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3. The phosphorylated β-catenin affects the protein association of β-catenin to E-cadherin and α-cateinin that occurs in the cell. This process can be reversed by de-phosphorylation of β-catenin.
Product Information
  • A431 cell lysate
    Included Positive Antigen Control: Catalog #12-301, Non stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
PresentationPurified rabbit polyclonal IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Quality LevelMQ100
ApplicationUse Anti-β-Catenin Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect β-Catenin also known as catenin (cadherin-associated protein) beta 1 (88kD).
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation:
5 μg of a previous lot immunoprecipitated β-Catenin from 500 μg of A431 membrane fraction lysate.

1-5 μg/mL of a previous lot showed positive immunostaining for β-catenin at cell-cell junctions of A431 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton® X-100.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected beta-Catenin in A431 and HUVEC cell lines.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide (CGG-SYLDSGIHSGATTTAPSLSGK) corresponding to the consensus GSK3 phosphorylation site of human β -Catenin (amino acids 29-49).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityRecognizes β-Catenin, Mr 92 kDa.
Species Reactivity
  • Human
  • Rat
  • Mouse
  • Rabbit
  • Bovine
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryBeta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
Gene Symbol
  • CTNNB1
  • Beta-catenin
  • PRO2286
  • FLJ37923
  • FLJ25606
  • DKFZp686D02253
Purification MethodProtein A chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P35222 # Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
SIZE: 781 amino acids; 85497 Da
SUBUNIT: Two separate pools are found in the cytoplasm: one is PSEN1/cadherin/catenin complex which anchors to the actin cytoskeleton. The other pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt- dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus, where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and DUPLIN. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with the PDZ domain of TAX1BP3; this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2. Interacts with SLC30A9 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 promotes nuclear translocation.
TISSUE SPECIFICITY: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheats. Expressed in colon.
PTM: Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr- 41 to Ser-37 and Ser-33. & EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. & Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X (Probable). Its ubiquitination leads to its subsequent proteasomal degradation.
DISEASE: "SwissProt: P35222 # Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life. & Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor. & Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. & A chromosomal rearrangement involving CTNNB1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1."
SIMILARITY: SwissProt: P35222 ## Belongs to the beta-catenin family. & Contains 12 ARM repeats.
Molecular Weight92 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on A431 total cell lysate.

Western Blot Analysis:
0.1-1 μg/mL of this lot detected β-catenin in an A431 total cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Prior to removing the cap, centrifuge the vial and gently mix the solution.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
06-734 04053252620423


Anti-β-Catenin Antibody SDS


Safety Data Sheet (SDS) 

Anti-β-Catenin Antibody Certificates of Analysis

TitleLot Number
Anti-#226;-Catenin - DAM1614918 DAM1614918
Anti-#946;-Catenin - 2016032 2016032
Anti-#946;-Catenin - DAM1810813 DAM1810813
Anti--Catenin - 2037638 2037638
Anti--Catenin - 2198989 2198989
Anti--Catenin - 2302182 2302182
Anti--Catenin - DAM1588240 DAM1588240
Anti--Catenin - DAM1713198 DAM1713198
Anti--Catenin - DAM1739165 DAM1739165
Anti--Catenin - DAM1782775 DAM1782775


Reference overviewApplicationSpeciesPub Med ID
Fluvastatin attenuates hepatic steatosis-induced fibrogenesis in rats through inhibiting paracrine effect of hepatocyte on hepatic stellate cells.
Chong, LW; Hsu, YC; Lee, TF; Lin, Y; Chiu, YT; Yang, KC; Wu, JC; Huang, YT
BMC gastroenterology  15  22  2015

Show Abstract
25886887 25886887
The impact of low-magnitude high-frequency vibration on fracture healing is profoundly influenced by the oestrogen status in mice.
Wehrle, E; Liedert, A; Heilmann, A; Wehner, T; Bindl, R; Fischer, L; Haffner-Luntzer, M; Jakob, F; Schinke, T; Amling, M; Ignatius, A
Disease models & mechanisms  8  93-104  2015

Show Abstract
Immunohistochemistry25381012 25381012
Role of TGF-β receptor III localization in polarity and breast cancer progression.
Meyer, AE; Gatza, CE; How, T; Starr, M; Nixon, AB; Blobe, GC
Molecular biology of the cell  25  2291-304  2014

Show Abstract
24870032 24870032
Aurora kinase a suppresses metabolic stress-induced autophagic cell death by activating mTOR signaling in breast cancer cells.
Xu, LZ; Long, ZJ; Peng, F; Liu, Y; Xu, J; Wang, C; Jiang, L; Guo, T; Kamran, M; Li, SS; Wang, CL; Wang, HJ; Zhao, YF; Wan, XY; Liu, Q
Oncotarget  5  7498-511  2014

Show Abstract
Western Blotting25115395 25115395
E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion.
McEwen, AE; Maher, MT; Mo, R; Gottardi, CJ
Molecular biology of the cell  25  2365-74  2014

Show Abstract
24966173 24966173
Therapeutic effect of a TM4SF5-specific monoclonal antibody against colon cancer in a mouse model.
Kim, YE; Kwon, S; Wu, G; Kim, D; Park, BK; Park, JA; Choi, KC; Kim, DS; Kwon, HJ; Lee, Y
Oncotarget  5  8402-15  2014

Show Abstract
25268742 25268742
The oncogenic properties of EWS/WT1 of desmoplastic small round cell tumors are unmasked by loss of p53 in murine embryonic fibroblasts.
Bandopadhayay, P; Jabbour, AM; Riffkin, C; Salmanidis, M; Gordon, L; Popovski, D; Rigby, L; Ashley, DM; Watkins, DN; Thomas, DM; Algar, E; Ekert, PG
BMC cancer  13  585  2013

Show Abstract
Immunohistochemistry (Paraffin)24321497 24321497
The intersection of genetic and chemical genomic screens identifies GSK-3α as a target in human acute myeloid leukemia.
Versha Banerji,Stacey M Frumm,Kenneth N Ross,Loretta S Li,Anna C Schinzel,Cynthia K Hahn,Rose M Kakoza,Kwan T Chow,Linda Ross,Gabriela Alexe,Nicola Tolliday,Haig Inguilizian,Ilene Galinsky,Richard M Stone,Daniel J Deangelo,Giovanni Roti,Jon C Aster,William C Hahn,Andrew L Kung,Kimberly Stegmaier
The Journal of clinical investigation  122  2012

Show Abstract
22326953 22326953
A DNA binding mutation in estrogen receptor-α leads to suppression of Wnt signaling via β-catenin destabilization in osteoblasts.
Mödder, UI; Rudnik, V; Liu, G; Khosla, S; Monroe, DG
Journal of cellular biochemistry  113  2248-55  2012

Show Abstract
22573547 22573547
Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis.
Liu, L; Alonso, V; Guo, L; Tourkova, I; Henderson, SE; Almarza, AJ; Friedman, PA; Blair, HC
The Journal of biological chemistry  287  43312-21  2012

Show Abstract
Western BlottingMouse23109343 23109343

Technical Info

Protein Blotting Handbook: 6th Edition

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Included Positive Control

Catalog Number Description
12-301 Non-Stimulated A431 Cell Lysate

Product Families


Life Science Research > Antibodies and Assays > Primary Antibodies