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PC679 Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb

PC679
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
PC679-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes the ~17 kDa cleaved fragment of caspase-3.
Catalogue NumberPC679
Brand Family Calbiochem®
SynonymsAnti-Active Caspase-3
Application Data
Detection of human cleaved caspase-3 by immunoblotting. Sample: Whole cell lysate from Jurkat cells treated with camptothecin (2 µM). Primary antibody: Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC679). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2, 30% glycerol, 0.5% BSA, 5 mM EDTA.
Positive controlA-431 cells treated with an inducer of apoptosis
Preservative0.03% Proclin
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesFrozen Sections (10-20 µg/ml)
Immunoblotting (0.5-1 µg/ml)
Immunocytochemistry (10-20 µg/ml)
Paraffin Sections (10-20 µg/ml, heat pre-treatment required)
Application CommentsInduction of caspase-3 can occur with many different treatments, such as treatment with 0.5 µg/ml actinomycin D for 24 h. Useful for the identification of apoptotic cells in tissue sections and populations of cells. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids surrounding the Asp¹⁷⁵ cleavage site of caspase-3
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PC679-50UG 07790788053987

Documentation

Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb Certificates of Analysis

TitleLot Number
PC679
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-January-2021 JSW
SynonymsAnti-Active Caspase-3
ApplicationFrozen Sections (10-20 µg/ml)
Immunoblotting (0.5-1 µg/ml)
Immunocytochemistry (10-20 µg/ml)
Paraffin Sections (10-20 µg/ml, heat pre-treatment required)
Application Data
Detection of human cleaved caspase-3 by immunoblotting. Sample: Whole cell lysate from Jurkat cells treated with camptothecin (2 µM). Primary antibody: Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC679). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~17 kDa cleaved fragment of caspase-3.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids surrounding the Asp¹⁷⁵ cleavage site of caspase-3
IsotypeIgG
Specieshuman, mouse, rat
Positive controlA-431 cells treated with an inducer of apoptosis
FormLiquid
FormulationIn PBS, pH 7.2, 30% glycerol, 0.5% BSA, 5 mM EDTA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative0.03% Proclin
CommentsInduction of caspase-3 can occur with many different treatments, such as treatment with 0.5 µg/ml actinomycin D for 24 h. Useful for the identification of apoptotic cells in tissue sections and populations of cells. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling