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681505 Anti-CaV2.2 (851-867) Rabbit pAb

Anti-CaV2.2 (851-867), Rabbit polyclonal, recognizes the ~210 and ~240 kDa forms of α1B-subunit of Cav2.2. It is validated for use in WB, ICC, IHC, and IP.

Anti-CaV2.2 (851-867) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-N-Type-Ca2+ Channel

681505
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
681505-1ST Plastic ampoule 1 st
Description
OverviewRecognizes the ~210 kDa and the ~240 kDa forms of α1B-subunit of Cav2.2. Supplied with a control peptide.
Note: The α-subunit is highly sensitive to proteases. Please refer to the product insert for proper sample preparation.
Catalogue Number681505
Brand Family Calbiochem®
SynonymsAnti-N-Type-Ca2+ Channel
Application Data
Detection of rat CaV2.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:200). Detection: chemiluminescence.

Detection of mouse of CaV2.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV2.2 channel (red) appearing in Purkinje cells (arrows) and distributed diffusely in the molecular layer (Mol) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) demonstrates the restruction of CaV2.2 to cell bodies but not to dendrites in the molecular layer (Figure B). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:100). Detection: fluorescence.
References
ReferencesWestenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, pH. 7.4 and 40 µg control peptide lyophilized from PBS.
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Application ReferencesImmunocytochemistry and Immunohistochemistry Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403. Immunoprecipitation Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Application NotesImmunoblotting (1.5-3 µg/ml)
Immunocytochemistry (see application references)
Immunohistochemistry (1:1000; see application references)
Immunoprecipitation (see application references)
Application CommentsThe epitope is specific for the α1B-subunit of the voltage-gated Ca2+ channel and is not present in any other known protein. Antibody binding can be fully inhibited with the soluble CP851-867 blocking peptide. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml) (Westenbroek, R.E., et al. 1995.). To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 minutes) to remove any aggregates. For use as a negative control, pre-incubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [(C)RHHRHRDRDKTSASTPA] corresponding to amino acids 851-867 of the α1B-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDissolve the antibody in 50 µl dH₂O to make a 300 µg/ml stock solution, aliquot and freeze (-20°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Dissolve the peptide in 100 µl dH₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Identification Number
Catalog Number GTIN
681505-1ST4055977260694

Documentation

Anti-CaV2.2 (851-867) Rabbit pAb Certificates of Analysis

TitleLot Number
681505

References

Reference overview
Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-November-2009 RFH
SynonymsAnti-N-Type-Ca2+ Channel
ApplicationImmunoblotting (1.5-3 µg/ml)
Immunocytochemistry (see application references)
Immunohistochemistry (1:1000; see application references)
Immunoprecipitation (see application references)
Application Data
Detection of rat CaV2.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:200). Detection: chemiluminescence.

Detection of mouse of CaV2.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV2.2 channel (red) appearing in Purkinje cells (arrows) and distributed diffusely in the molecular layer (Mol) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) demonstrates the restruction of CaV2.2 to cell bodies but not to dendrites in the molecular layer (Figure B). Primary antibody: Anti-CaV2.2 (851-867) Rabbit pAb (Cat. No. 681505) (1:100). Detection: fluorescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~210 kDa and the ~240 kDa forms the voltage-gated Ca2+ channel of α1B-subunit. Supplied with a control peptide (immunogen).
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide [(C)RHHRHRDRDKTSASTPA] corresponding to amino acids 851-867 of the α1B-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
IsotypeIgG
Speciesmouse, rat
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, pH. 7.4 and 40 µg control peptide lyophilized from PBS.
Preservative≤0.1% sodium azide (antibody only)
CommentsThe epitope is specific for the α1B-subunit of the voltage-gated Ca2+ channel and is not present in any other known protein. Antibody binding can be fully inhibited with the soluble CP851-867 blocking peptide. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml) (Westenbroek, R.E., et al. 1995.). To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 minutes) to remove any aggregates. For use as a negative control, pre-incubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDissolve the antibody in 50 µl dH₂O to make a 300 µg/ml stock solution, aliquot and freeze (-20°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Dissolve the peptide in 100 µl dH₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesWestenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
Westenbroek, R.E., et al. 1992. Neuron 9, 1099.
Application referencesImmunocytochemistry and Immunohistochemistry Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403. Immunoprecipitation Westenbroek, R.E., et al. 1992. Neuron 9, 1099.