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MABF981 Anti-CXCR4 Antibody, clone 12G5

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MABF981
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, NEUTMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF981
DescriptionAnti-CXCR4 Antibody, clone 12G5
Alternate Names
  • C-X-C chemokine receptor type 4
  • CXC-R4
  • CXCR-4
  • FB22
  • HM89
  • LCR1
  • Leukocyte-derived seven transmembrane domain receptor
  • LESTR
  • Lipopolysaccharide-associated protein 3
  • LAP-3
  • LPS-associated protein 3
  • NPYRL
  • Stromal cell-derived factor 1 receptor
  • SDF-1 receptor
  • CXCR4
  • Fusin
  • CD184
Background InformationC-X-C chemokine receptor type 4 (UniProt P61073; also known as CD184, CXC-R4, CXCR-4, FB22, Fusin, HM89, LAP-3, LCR1, LESTR, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, LPS-associated protein 3, Neuropeptide Y receptor Y3, NPYRL, SDF-1 receptor, Stromal cell-derived factor 1 receptor) is encoded by the CXCR4 (also known as D2S201E, HSY3RR, LAP3, NPY3R, NPYR, NPYY3R, WHIM, WHIMS) gene (Gene ID 7592) in human. Chemokines and their receptors direct migration of distinct leukocyte subsets to sites of inflammation and to their specific niches in lymphoid organs. Virtually all T cell chemoattractants selectively attract memory/activated T cells. CXCR4 is the seven-transmembrane receptor for SDF-1 that is essential for the development of non-hematopoietic tissues, including heart and B lymphocytes. CXCR4 and CCR5 are two chemokine receptors that also function as co-receptors for human immunodeficiency virus (HIV) entry into CD4+ cells. During the early stages of HIV infection, viral isolates tend to use CCR5 for viral entry, while later isolates tend to use CXCR4.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-CXCR4 Antibody, clone 12G5 is validated for use in Flow Cytometry, Immunocytochemistry and Neutralizing for the detection of CXCR4.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Neutralizing
Application NotesFlow Cytometry Analysis: A representative lot detected surface expression of human CXCR4 in various cell types by flow cytometry (Bleul, C.C., et al. (1997). Proc. Natl. Acad. Sci. U S A.94(5):1925-1930; McKnight, A., et al. (1997). J. Virol. 71(2):1692-1696; Endres, M.J., et al. (1996). Cell. 87(4):745-756)
Immunocytochemistry Analysis: A representative lot stained paraformaldehyde-fixed CHO cells transfected to stably express human CXCR4/fusin (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
Neutralizing Analysis: A representative lot blocked SDF-1-induced lymphocyte chemotaxis using freshly isolated human PBMCs (Bleul, C.C., et al. (1997). Proc. Natl. Acad. Sci. U S A.94(5):1925-1930).
Neutralizing Analysis: A representative lot blocked the infection of CXCR4+/CD4+ rhabdomyosarcoma (RD) cells by seven HIV-1 (LAI, RF, Gun-1wt, Gun-1var) and HIV-2 (ROD A/B and CBL-23) strains as assessed by >95% reduced syncytium (multinucleated cell) formation due to viral infection-induced cell-to-cell fusion. Clone 12G5 also blocked Gun-1wt, Gun-1var, and CBL-23, but not the other 4 HIV strains from infecting HeLa/CD4 and C8166 cultures (McKnight, A., et al. (1997). J. Virol. 71(2):1692-1696).
Neutralizing Analysis: A representative lot blocked HIV-2 /vcp strain from infecting U87 cells transfected to express human CXCR4/fusin as assessed a reduction of syncytium (multinucleated cell) formation due to viral infection-induced cell-to-cell fusion (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
Note: Although clone 12G5 binds cell surface CXCR4, efforts to immunoprecipitate or immunoblot CXCR4 were reported to be unsuccessful (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
Biological Information
ImmunogenSimian immunodeficiency virus (SIVmac) variant CP-MAC-infected Sup-T1 human T lymphoblasts.
EpitopeExtracellular domain
Clone12G5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CXCR4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~39 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CXCR4 in Jurkat cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF981 04055977294170

Documentation

Anti-CXCR4 Antibody, clone 12G5 SDS

Title

Safety Data Sheet (SDS) 

Anti-CXCR4 Antibody, clone 12G5 Certificates of Analysis

TitleLot Number
Anti-CXCR4, clone 12G5 - 3324184 3324184
Anti-CXCR4, clone 12G5 - 3389936 3389936
Anti-CXCR4, clone 12G5 -Q2610505 Q2610505
Anti-CXCR4, clone 12G5 Monoclonal Antibody 3143108

References

Reference overviewPub Med ID
Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent.
McKnight, A; Wilkinson, D; Simmons, G; Talbot, S; Picard, L; Ahuja, M; Marsh, M; Hoxie, JA; Clapham, PR
Journal of virology  71  1692-6  1997

Show Abstract
8995702 8995702
The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.
Bleul, CC; Wu, L; Hoxie, JA; Springer, TA; Mackay, CR
Proceedings of the National Academy of Sciences of the United States of America  94  1925-30  1997

Show Abstract
9050881 9050881
CD4-independent infection by HIV-2 is mediated by fusin/CXCR4.
Endres, MJ; Clapham, PR; Marsh, M; Ahuja, M; Turner, JD; McKnight, A; Thomas, JF; Stoebenau-Haggarty, B; Choe, S; Vance, PJ; Wells, TN; Power, CA; Sutterwala, SS; Doms, RW; Landau, NR; Hoxie, JA
Cell  87  745-56  1996

Show Abstract
8929542 8929542

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Title
Post-Translational Modifications