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MABC951 Anti-CXCL16/SR-PSOX Antibody, clone 22-19-12

MABC951
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, InhibitionMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC951
      DescriptionAnti-CXCL16/SR-PSOX Antibody, clone 22-19-12
      Alternate Names
      • C-X-C motif chemokine 16
      • CXC chemokine ligand 16
      • Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
      • Small-inducible cytokine B16
      • SR-PSOX
      • Transmembrane chemokine CXCL16
      Background InformationC-X-C motif chemokine 16 (UniProt Q9H2A7; also known as CXC chemokine ligand 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, SR-PSOX, Transmembrane chemokine CXCL16) is encoded by the CXCL16 (also known as CXCLG16, SCYB16, SRPSOX, UNQ2759/PRO6714) gene (Gene ID 58191) in human. CXCL16 functions as the ligand for the chemokine receptor CXCR6 and is one of two chemokines known to exist in both transmembrane and soluble forms. CXCL16 is expressed on leukocytes (macrophages, dendritic cells, B-cells, and monocytes), epidermal keratinocytes, bronchial epithelial cells and renal podocytes. CXCL16 mediates the recruitment and adhesion of CXCR6-expressing cells. In addition to leucocyte recruitment and inflammation, CXCR6 and CXCL16 are also reported to be involved in angiogenesis and their expression shows both positive and negative correlations with cancer patients survival. CXCL16 is initially produced with a signal peptide (a.a. 1-29), the removal of which yields the mature protein with a large extracellular region (a.a. 30-205), a trarnsmembrane segment (a.a. 206-226), and a small cytoplasmic tail (a.a. 227-254). Its extracellular region contains a mucin-like stalk and the CXC chemokine domain (a.a. 32-107) that mediates receptor interaction.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CXCL16/SR-PSOX Antibody, clone 22-19-12 is an antibody against CXCL16 for use in Flow Cytometry, Inhibition.
      Key Applications
      • Flow Cytometry
      • Inhibition
      Application NotesFlow Cytometry Analysis: A representative lot detected CXCL16/SR-PSOX surface expression on myeloid antigen presenting cells (APCs), including monocytes, macrophages, CD11c+ myeloid dendritic cells (mDCs), monocyte-derived DCs (MoDCs), while no CXCL16/SR-PSOX surface expression was found on plasmacytoid dendritic cells (pDCs) (Tabata, S., et al. (2005). J. Leukoc. Biol. 77(5):777-786).
      Flow Cytometry Analysis: A representative lot detected CXCL16/SR-PSOX surface expression on human PBMC-derived dendritic cells (DCs) and PMA-treated human acute monocytic leukemia THP-1 cells, as well as the exogenously expressed human PSOX/CXCL16 on transfected COS-7 cells (Shimaoka, T., et al. (2003). J. Immunol. 171(4):1647-1651).
      Neutralizing Analysis: The F(ab)'2 fragment from a representative lot neutralized the chemotactic activity of recombinant CXCL16/SR-PSOX extracellular domain-SEAP fusion toward CXCR6-expressing L1.2 cells (Shimaoka, T., et al. (2003). J. Immunol. 171(4):1647-1651).
      Neutralizing Analysis: The F(ab)'2 fragment from a representative lot inhibited CXCL16/SR-PSOX-dependent bacterial phagocytosis by human PBMC-derived dendritic cells (DCs), PMA-treated human acute monocytic leukemia THP-1 cells, as well as human CXCL16/SR-PSOX-expressing COS-7 cells (Shimaoka, T., et al. (2003). J. Immunol. 171(4):1647-1651).
      Note: When conducting neutralization or flow cytometric analysis of cells with surface Fc receptor, use F(ab)'2 fragment or preblock cell surface Fc receptor to avoid primary antibody binding to the Fc receptor.
      Biological Information
      ImmunogenHuman IgG1 Fc-tagged recombinant human CXCL16/SR-PSOX extracellular fragment.
      EpitopeChemokine domain.
      Clone22-19-12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 22-19-12 neutralizes human CXCL16/SR-PSOX-mediated chemotactic and phagocytosis activities by targeting the exracellular chemokine domain (Shimaoka, T., et al. (2003). J. Immunol. 171(4):1647-1651).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CXCL16
      • CXCLG16
      • SCYB16
      • SRPSOX
      • UNQ2759/PRO6714
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight24.405 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in PMA-treated THP-1 human monocytes.

      Flow Cytometry Analysis: 0.1 µg of this antibody detected CXCL16/SR-PSOX surface expression on PMA-treated THP-1 human monocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications