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AB3006 Anti-CREB Antibody

AB3006
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, EMSA, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3006
      Replaces04-218
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CREB Antibody
      Alternate Names
      • CREB-1
      • cAMP-response Element-binding Protein-1
      Background InformationCREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.
      References
      Product Information
      FormatPurified
      Control
      • Forskolin-treated PC12 cell lysate
      PresentationPurified immunoglobulin. Liquid (sterile filtered) in 0.02M Potassium Phosphate buffer containing 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-CREB Antibody (Rabbit Polyclonal Antibody) validated in ELISA, EMSA, IP, WB to detect CREB also known as CREB-1, cAMP-response Element-binding Protein-1.
      Key Applications
      • ELISA
      • Electrophoretic Mobility Shift Assay
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoblot

      Immunoprecipitation

      ELISA

      Supershift assay: 0.5-1.0 μL per assay.

      Optimal dilutions must be determined by the end user.
      Biological Information
      ImmunogenCREB-1 (p43) peptide corresponding to the N-terminal (aa 5-45) of human protein conjugated to KLH.
      HostRabbit
      SpecificityHuman cyclic AMP responsive element binding protein-1 (CREB-1, p43). By Western blot it shows a major band at 46 kDa when assayed against a lysate of 1 x 106 Raji B cells. Minor bands at 75 kDa and 43 kDa may be noted. Partial reactivity to CREM-1 protein. May react non-specifically with other proteins.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
      Gene Symbol
      • CREB1
      • MGC9284
      • CREB
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.
      SIZE: 341 amino acids; 36688 Da
      SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CREB Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CREB Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-CREB-1 (p43) 2438244
      RABBIT ANTI-CREB-1 (p43) POLYCLONAL ANTIBODY - 2025960 2025960
      RABBIT ANTI-CREB-1 (p43) - 3252122 3252122
      RABBIT ANTI-CREB-1 (p43) - 3774188 3774188
      RABBIT ANTI-CREB-1 (p43) POLYCLONAL ANTIBODY 2941586
      RABBIT ANTI-CREB-1 (p43) POLYCLONAL ANTIBODY - 2201411 2201411
      RABBIT ANTI-CREB-1 (p43) POLYCLONAL ANTIBODY - 2322546 2322546
      RABBIT ANTI-CREB-1 (p43) POLYCLONAL ANTIBODY -2502909 2502909

      Data Sheet

      Title
      RABBIT ANTI-CREB-1 (p43) POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies