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AB1847 Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop

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100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbPurifiedPolyclonal Antibody
Catalogue NumberAB1847
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop
Alternate Names
  • CD184
  • CXCR4
Product Information
PresentationPurified IgG in PBS containing 0.02% sodium azide.
Quality LevelMQ100
ApplicationAnti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop detects level of C-X-C Chemokine Receptor 4 & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:500 - 1000

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenExtracellular loop peptide of human CXCR4 (Berson et al. 1996; Leotscher et al. 1994).
Epitopeextracellular loop
SpecificitySome G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (Nimitrov 1997). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principle coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (Feng et al. 1996; Berson et al. 1996). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (Doranz et al. 1996; Endres et al. 1996). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (Bleul et al. 1996; Oberlin et al. 1996). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (Lapham et al. 1996). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (Feng et al. 1996). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (Feng et al. 1996; Endres et al. 1996; Brelot et al. 1997). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (Brelot et al. 1997; Lu et al. 1997).
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene Symbol
  • CXCR4
  • WHIM
  • NPY3R
  • NPYY3R
  • FB22
  • LAP3
  • Fusin
  • CXCR-4
  • LCR1
  • NPYR
  • HM89
  • fusin
  • HSY3RR
  • CXC-R4
  • NPYY3
  • D2S201E
  • CD184
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P61073 # Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
SIZE: 352 amino acids; 39746 Da
SUBUNIT: Interacts with HIV-1 surface protein gp120 and Tat.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
DOMAIN: SwissProt: P61073 The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
PTM: Sulfated.
DISEASE: SwissProt: P61073 # Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also called warts, hypogammaglobulinemia, infections, and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsCan be refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
AB1847 04053252274251


Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop SDS


Safety Data Sheet (SDS) 

Anti-C-X-C Chemokine Receptor 4 Antibody, extracellular loop Certificates of Analysis

TitleLot Number


Reference overviewPub Med ID
Akt/hypoxia-inducible factor-1α signaling deficiency compromises skin wound healing in a type 1 diabetes mouse model.
Jing, L; Li, S; Li, Q
Experimental and therapeutic medicine  9  2141-2146  2015

Show Abstract
26136949 26136949
Chemical Library Screening and Structure-Function Relationship Studies Identify Bisacodyl as a Potent and Selective Cytotoxic Agent Towards Quiescent Human Glioblastoma Tumor Stem-Like Cells.
Zeniou, M; Fève, M; Mameri, S; Dong, J; Salomé, C; Chen, W; El-Habr, EA; Bousson, F; Sy, M; Obszynski, J; Boh, A; Villa, P; Assad Kahn, S; Didier, B; Bagnard, D; Junier, MP; Chneiweiss, H; Haiech, J; Hibert, M; Kilhoffer, MC
PloS one  10  e0134793  2015

Show Abstract
26270679 26270679
CXCR4 attenuates cardiomyocytes mitochondrial dysfunction to resist ischaemia-reperfusion injury.
Cai, WF; Kang, K; Huang, W; Liang, JL; Feng, YL; Liu, GS; Chang, DH; Wen, ZL; Paul, C; Xu, M; Millard, RW; Wang, Y
Journal of cellular and molecular medicine  19  1825-35  2015

Show Abstract
25824297 25824297
Effects of progesterone on the content of CCR5 and CXCR4 coreceptors in PBMCs of seropositive and exposed but uninfected Mexican women to HIV-1.
Edith Cabrera-Muñoz,Luis L Fuentes-Romero,Jorge Zamora-Chávez,Ignacio Camacho-Arroyo,Luis E Soto-Ramírez
The Journal of steroid biochemistry and molecular biology  132  2012

Show Abstract
22342838 22342838
CD24 affects CXCR4 function in pre-B lymphocytes and breast carcinoma cells.
Schabath, H; Runz, S; Joumaa, S; Altevogt, P
Journal of cell science  119  314-25  2006

Show Abstract
16390867 16390867
Role of the first and third extracellular domains of CXCR-4 in human immunodeficiency virus coreceptor activity.
Brelot, A, et al.
J. Virol., 71: 4744-51 (1997)  1997

Show Abstract
9151868 9151868
Evolution of HIV-1 coreceptor usage through interactions with distinct CCR5 and CXCR4 domains.
Lu, Z, et al.
Proc. Natl. Acad. Sci. U.S.A., 94: 6426-31 (1997)  1997

Show Abstract
9177234 9177234
How do viruses enter cells? The HIV coreceptors teach us a lesson of complexity.
Dimitrov, D S
Cell, 91: 721-30 (1997)  1997

9413981 9413981
HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor.
Feng, Y, et al.
Science, 272: 872-7 (1996)  1996

Show Abstract
8629022 8629022
A dual-tropic primary HIV-1 isolate that uses fusin and the beta-chemokine receptors CKR-5, CKR-3, and CKR-2b as fusion cofactors.
Doranz, B J, et al.
Cell, 85: 1149-58 (1996)  1996

Show Abstract
8674120 8674120

Data Sheet


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Life Science Research > Antibodies and Assays > Primary Antibodies