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AB1846 Anti-C-X-C Chemokine Receptor 4 Antibody, NT

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100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IP, WBRbPurifiedPolyclonal Antibody
Catalogue NumberAB1846
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-C-X-C Chemokine Receptor 4 Antibody, NT
Alternate Names
  • CD184
  • CXCR4
Product Information
  • Skin
PresentationPurified IgG in PBS containing 0.02% sodium azide.
Quality LevelMQ100
ApplicationAnti-C-X-C Chemokine Receptor 4 Antibody, N-terminus is an antibody against C-X-C Chemokine Receptor 4 for use in IC, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern blot: 1-2 μg/mL. HeLa cell lysates can be used as positive control. The antibody should detect a band at 40 kDa.

Immunocytochemistry: 10 μg/mL


Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenAmino-terminus peptide of human CXCR4 (Berson, 1996; Leotscher, 1994).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityRecognizes CXCR4, N-terminus.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene Symbol
  • CXCR4
  • WHIM
  • NPY3R
  • NPYY3R
  • FB22
  • LAP3
  • Fusin
  • CXCR-4
  • LCR1
  • NPYR
  • HM89
  • fusin
  • HSY3RR
  • CXC-R4
  • NPYY3
  • D2S201E
  • CD184
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P61073 # Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
SIZE: 352 amino acids; 39746 Da
SUBUNIT: Interacts with HIV-1 surface protein gp120 and Tat.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
DOMAIN: SwissProt: P61073 The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
PTM: Sulfated.
DISEASE: SwissProt: P61073 # Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also called warts, hypogammaglobulinemia, infections, and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
Molecular Weight40 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain 1 year at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
AB1846 04053252368936


Anti-C-X-C Chemokine Receptor 4 Antibody, NT SDS


Safety Data Sheet (SDS) 

Anti-C-X-C Chemokine Receptor 4 Antibody, NT Certificates of Analysis

TitleLot Number
ANTI-CXCR4 -2691395 2691395
RABBIT ANTI-CXCR4 (N-TERMINUS) - 3466655 3466655
RABBIT ANTI-CXCR4 (N-TERMINUS) - 4097248 4097248
RABBIT ANTI-CXCR4 (N-TERMINUS) -2716580 2716580


Reference overviewApplicationPub Med ID
Effects and mechanisms of blocking the hedgehog signaling pathway in human gastric cancer cells.
Gu, H; Li, XU; Zhou, C; Wen, Y; Shen, Y; Zhou, L; Li, J
Oncology letters  9  1997-2002  2015

Show Abstract
26137001 26137001
Tyrosylprotein sulfotransferase-1 and tyrosine sulfation of chemokine receptor 4 are induced by Epstein-Barr virus encoded latent membrane protein 1 and associated with the metastatic potential of human nasopharyngeal carcinoma.
Xu, J; Deng, X; Tang, M; Li, L; Xiao, L; Yang, L; Zhong, J; Bode, AM; Dong, Z; Tao, Y; Cao, Y
PloS one  8  e56114  2013

Show Abstract
Western Blotting23472069 23472069
Androgens Induce Functional CXCR4 through ERG Factor Expression in TMPRSS2-ERG Fusion-Positive Prostate Cancer Cells.
Cai, Juan, et al.
Transl Oncol, 3: 195-203 (2010)  2010

Show Abstract
20563261 20563261
Nitric oxide donor upregulation of stromal cell-derived factor-1/chemokine (CXC motif) receptor 4 enhances bone marrow stromal cell migration into ischemic brain after stroke.
Cui, X; Chen, J; Zacharek, A; Li, Y; Roberts, C; Kapke, A; Savant-Bhonsale, S; Chopp, M
Stem cells (Dayton, Ohio)  25  2777-85  2007

Show Abstract
Fluorescence Activated Cell Sorting (FACS)17641243 17641243
Differential functional activation of chemokine receptor CXCR4 is mediated by G proteins in breast cancer cells
Holland, Jane D, et al
Cancer Res, 66:4117-24 (2006)  2006

16618732 16618732
Stromal cell-derived factor 1alpha mediates neural progenitor cell motility after focal cerebral ischemia.
Robin, AM; Zhang, ZG; Wang, L; Zhang, RL; Katakowski, M; Zhang, L; Wang, Y; Zhang, C; Chopp, M
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  26  125-34  2006

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15959456 15959456
Transactivation of CXCR4 by the insulin-like growth factor-1 receptor (IGF-1R) in human MDA-MB-231 breast cancer epithelial cells
Akekawatchai, Chareeporn, et al
J Biol Chem, 280:39701-8 (2005)  2005

16172123 16172123
CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow.
Avigdor, A; Goichberg, P; Shivtiel, S; Dar, A; Peled, A; Samira, S; Kollet, O; Hershkoviz, R; Alon, R; Hardan, I; Ben-Hur, H; Naor, D; Nagler, A; Lapidot, T
Blood  103  2981-9  2004

Show Abstract
15070674 15070674
Role of the first and third extracellular domains of CXCR-4 in human immunodeficiency virus coreceptor activity.
Brelot, A, et al.
J. Virol., 71: 4744-51 (1997)  1997

Show Abstract
9151868 9151868
Evolution of HIV-1 coreceptor usage through interactions with distinct CCR5 and CXCR4 domains.
Lu, Z, et al.
Proc. Natl. Acad. Sci. U.S.A., 94: 6426-31 (1997)  1997

Show Abstract
9177234 9177234

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Life Science Research > Antibodies and Assays > Primary Antibodies