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09-143 Anti-Brk Antibody

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09-143
50 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number09-143
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Brk Antibody
Alternate Names
  • PTK6
  • protein tyrosine kinase 6
  • breast tumor kinase
  • protein-tyrosine kinase BRK
References
Product Information
FormatAffinity Purified
Presentation50 µg of affinity purified rabbit polyclonal IgG in 0.02 M PBS, 250 mM sodium chloride containing 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Brk using this Anti-Brk Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH conjugated synthetic peptide corresponding to the N-terminal region of human Brk.
HostRabbit
SpecificityRecognizes Brk (breast tumor kinase, PTK6) at approximately 52 kDa. It can also recognize the 73 kDa ELF-related protein that shares 70% homology with the immunizing peptide.
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation.
Gene Symbol
  • PTK6
  • BRK
  • FLJ42088
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q13882 # Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
SIZE: 451 amino acids; 51834 Da
SUBUNIT: Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
TISSUE SPECIFICITY: Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PTM: Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
SIMILARITY: SwissProt: Q13882 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight52 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore for 1 year at 2-8°C from date of shipment.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
09-143 04053252736766

Documentation

Anti-Brk Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Brk Antibody Certificates of Analysis

TitleLot Number
Anti-Brk (Affinity Purified Rabbit Polyclonal IgG) - 2399454 2399454
Anti-Brk (Affinity Purified Rabbit Polyclonal IgG) 2869706
Anti-Brk (Affinity Purified Rabbit Polyclonal IgG) 2868378
Anti-Brk (Affinity Purified Rabbit Polyclonal IgG) - 3378622 3378622
Anti-Brk - 2530946 2530946
Anti-Brk - 3179970 3179970
Anti-Brk - DAM1576945 DAM1576945
Anti-Brk - DAM1597375 DAM1597375
Anti-Brk - DAM1634449 DAM1634449
Anti-Brk - DAM1644571 DAM1644571

References

Reference overviewPub Med ID
Growth response of Escherichia coli ATCC 35218 adapted to several concentrations of sodium benzoate and potassium sorbate.
Santiesteban-López NA, Rosales M, Palou E, López-Malo A
J Food Prot  72  2301-7.  2009

Show Abstract
19903392 19903392
Expression and oncogenic role of Brk (PTK6/Sik) protein tyrosine kinase in lymphocytes
Kasprzycka, Monika, et al
Am J Pathol, 168:1631-41 (2006)  2006

16651629 16651629
The intracellular tyrosine kinase Brk sensitizes non-transformed cells to inducers of apoptosis
Haegebarth, Andrea, et al
Cell Cycle, 4:1239-46 (2005)  2005

16082217 16082217
Interaction between Brk kinase and insulin receptor substrate-4
Qiu, Haoqun, et al
Oncogene, 24:5656-64 (2005)  2005

15870689 15870689
Regulation of the nonreceptor tyrosine kinase Brk by autophosphorylation and by autoinhibition
Qiu, Haoqun and Miller, W Todd
J Biol Chem, 277:34634-41 (2002)  2002

12121988 12121988