AB5613P Anti-Brain Derived Neurotrophic Factor Antibody, pro

AB5613P
20 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5613P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Brain Derived Neurotrophic Factor Antibody, pro
      Alternate Names
      • Prepro-Abrineurin
      • Pro-Abrineurin
      • Prepro-BDNF
      • Pro-BDNF
      • Prepro-Brain-derived neurotrophic factor
      • Pro-Brain-derived neurotrophic factor
      Background InformationBrain-derived neurotrophic factor (UniProt P23560; also known as Abrineurin, BDNF, Neurotrophin) is encoded by the BDNF (also known as BULN2) gene (Gene ID 627) in human. BDNF is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT3, and NT4/5. BDNF mediates its neurotrophic properties by signaling through the high affinity cell surface receptor gp145/trkB. BDNF is initially produced with a signal peptide (a.a. 1-18) and a propeptide (a.a. 19-128) sequence, the removal of which yields the mature BDNF (a.a. 129-247).
      References
      Product Information
      FormatAffinity Purified
      PresentationLyophilized from 10 mM PBS. Reconstitute with 20 μL of sterile distilled water.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Brain Derived Neurotrophic Factor Antibody, pro is an antibody proBDNF for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:2,000

      Immunohistochemistry: 1:2,000

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region within human BDNF propeptide sequence.
      EpitopePropeptide.
      HostRabbit
      SpecificityThis polyclonal antibody detects prepro- and pro-BDNF, as well as prepro-/pro-BDNF-derived N-terminal fragments that contain the propeptide sequence (a.a. 19-128), but not mature BDNF or C-terminal fragments without the propeptide sequence. No cross-reactivity to other proneurotrophins.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BDNF
      UniProt Number
      Molecular Weight~35 kDa (glycosylated prepro-/pro-BDNF), ~50-65 kDa (glycosylated prepro-/pro-BDNF dimer), and ~26-28 kDa (non-glycosylated prepro/pro-BDNF or glycosylated prepro-/pro-BDNF N-terminal fragments) observed. Target bands appear larger than the calculated molecular weights of 27.82/28.87/29.82/37.12/31.12 kDa (human prepro-BDNF isoform 1/2/3/4/5) and 25.77/26.82/27.77/35.07/29.06 kDa (human pro-BDNF isoform 1/2/3/4/5) due to glycosylation and/or dimerization. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C undiluted for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.
      Packaging Information
      Material Size20 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Brain Derived Neurotrophic Factor Antibody, pro SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Brain Derived Neurotrophic Factor Antibody, pro Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-proBDNF AFFINITY PURIFIED POLYCLONAL ANTIBODY - 21189052118905
      RABBIT ANTI-proBDNF AFFINITY PURIFIED POLYCLONAL ANTIBODY - 21365962136596
      RABBIT ANTI-proBDNF AFFINITY PURIFIED POLYCLONAL ANTIBODY - 21474142147414
      RABBIT ANTI-proBDNF - 32779813277981
      RABBIT ANTI-proBDNF -26531302653130
      RABBIT ANTI-proBDNF AFFINITY PURIFIED POLYCLONAL ANTIBODY3048313
      RABBIT ANTI-proBDNF AFFINITY PURIFIED POLYCLONAL ANTIBODY - 22838132283813

      References

      Reference overviewApplicationPub Med ID
      Exogenous t-PA administration increases hippocampal mature BDNF levels. plasmin- or NMDA-dependent mechanism?
      Rodier, M; Prigent-Tessier, A; Béjot, Y; Jacquin, A; Mossiat, C; Marie, C; Garnier, P
      PloS one  9  e92416  2014

      Show Abstract
      24670989 24670989
      Comparative effect of treadmill exercise on mature BDNF production in control versus stroke rats.
      Quirié, A; Hervieu, M; Garnier, P; Demougeot, C; Mossiat, C; Bertrand, N; Martin, A; Marie, C; Prigent-Tessier, A
      PloS one  7  e44218  2012

      Show Abstract
      22962604 22962604
      Motoneuron programmed cell death in response to proBDNF.
      Taylor, AR; Gifondorwa, DJ; Robinson, MB; Strupe, JL; Prevette, D; Johnson, JE; Hempstead, B; Oppenheim, RW; Milligan, CE
      Developmental neurobiology  72  699-712  2012

      Show Abstract
      21834083 21834083
      Dysregulation of BDNF-TrkB signaling in developing hippocampal neurons by Pb(2+): implications for an environmental basis of neurodevelopmental disorders.
      Stansfield, KH; Pilsner, JR; Lu, Q; Wright, RO; Guilarte, TR
      Toxicological sciences : an official journal of the Society of Toxicology  127  277-95  2012

      Show Abstract
      Immunocytochemistry22345308 22345308
      Prenatal stress differentially alters brain-derived neurotrophic factor expression and signaling across rat strains.
      Neeley, EW; Berger, R; Koenig, JI; Leonard, S
      Neuroscience  187  24-35  2011

      Show Abstract
      Western Blotting21497180 21497180
      Lead exposure during synaptogenesis alters vesicular proteins and impairs vesicular release: potential role of NMDA receptor-dependent BDNF signaling.
      Neal, AP; Stansfield, KH; Worley, PF; Thompson, RE; Guilarte, TR
      Toxicological sciences : an official journal of the Society of Toxicology  116  249-63  2010

      Show Abstract
      Immunocytochemistry20375082 20375082
      Brain-derived neurotrophic factor rescues and prevents chronic intermittent hypoxia-induced impairment of hippocampal long-term synaptic plasticity.
      Hui Xie,Kin-Ling Leung,Lei Chen,Ying-Shing Chan,Pak-Cheung Ng,Tai-Fai Fok,Yun-Kwok Wing,Ya Ke,Albert M Li,Wing-Ho Yung
      Neurobiology of disease  40  2010

      Show Abstract
      20553872 20553872
      Fetal alcohol spectrum disorder-associated depression: evidence for reductions in the levels of brain-derived neurotrophic factor in a mouse model.
      Caldwell, KK; Sheema, S; Paz, RD; Samudio-Ruiz, SL; Laughlin, MH; Spence, NE; Roehlk, MJ; Alcon, SN; Allan, AM
      Pharmacology, biochemistry, and behavior  90  614-24  2008

      Show Abstract
      18558427 18558427
      Locomotor exercise alters expression of pro-brain-derived neurotrophic factor, brain-derived neurotrophic factor and its receptor TrkB in the spinal cord of adult rats.
      Matylda Macias,Anna Dwornik,Ewelina Ziemlinska,Susanna Fehr,Melitta Schachner,Julita Czarkowska-Bauch,Malgorzata Skup
      The European journal of neuroscience  25  2007

      Show Abstract
      17445239 17445239

      Data Sheet

      Title
      RABBIT ANTI-proBDNF

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      Catalog Number Description  
      AB5613 Anti-Brain Derived Neurotrophic Factor Antibody, pro Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies