AB1779SP Anti-Brain Derived Neurotrophic Factor Antibody

AB1779SP
500 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R ELISA, WB, IHC Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1779SP
      ReplacesAB1534P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Brain Derived Neurotrophic Factor Antibody
      Alternate Names
      • BDNF
      References
      Product Information
      FormatPurified
      PresentationImmunoglobulin fraction lyophilized from PBS, with no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
      Quality LevelMQ100
      Applications
      ApplicationAnti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, WB, IH.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 5-10μg/mL (see suggested protocol).

      Immunoblotting: 5-10μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C. BDNF migrates as 18kDa, with a homodimer at ~27-30kDa. 1:1,000

      ELISA: 5-10μg/mL

      BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.



      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant Human Brain Derived Neurotrophic Factor (BDNF).
      HostRabbit
      SpecificityBrain Derived Neurotrophic Factor (BDNF). By dot blot, less than 1% cross-reactivity against NGF, NT3 or NT4.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
      Gene Symbol
      • BDNF
      • Abrineurin
      • neurotrophin
      • MGC34632
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
      SIZE: 247 amino acids; 27818 Da
      SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
      SUBCELLULAR LOCATION: Secreted.
      PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
      DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
      SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20 to -70°C for up to one year after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Glycerol (ACS grade or better) can be added (1:1) for additional stability.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Brain Derived Neurotrophic Factor Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY - 24645032464503
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) - 31991793199179
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) - 32097183209718
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) - 32319223231922
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) - 33184943318494
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY3117778
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY3114523
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY3099566
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY2930539
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY2907610

      References

      Reference overviewApplicationPub Med ID
      BDNF overexpression in the ventral tegmental area prolongs social defeat stress-induced cross-sensitization to amphetamine and increases ΔFosB expression in mesocorticolimbic regions of rats.
      Wang, J; Fanous, S; Terwilliger, EF; Bass, CE; Hammer, RP; Nikulina, EM
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  38  2286-96  2013

      Show Abstract
      23689674 23689674
      Chronic ketamine administration modulates midbrain dopamine system in mice.
      Tan, S; Lam, WP; Wai, MS; Yu, WH; Yew, DT
      PloS one  7  e43947  2012

      Show Abstract
      Western Blotting22937133 22937133
      Sensitized activation of Fos and brain-derived neurotrophic factor in the medial prefrontal cortex and ventral tegmental area accompanies behavioral sensitization to amphetamine.
      Fanous S, Lacagnina MJ, Nikulina EM, Hammer RP Jr.
      Neuropharmacology  61  558-64  2011

      Show Abstract
      21570990 21570990
      Noradrenaline activation of neurotrophic pathways protects against neuronal amyloid toxicity.
      Scott E Counts,Elliott J Mufson
      Journal of neurochemistry  113  2010

      Show Abstract Full Text Article
      20132474 20132474
      Effects of BDNF infusion on the axon terminals of locus coeruleus neurons of aging rats.
      Sadamu Nakai, Wataru Matsunaga, Yoshiyuki Ishida, Ken-ichi Isobe, Tetsuya Shirokawa
      Neuroscience research  54  213-9  2006

      Show Abstract
      16406148 16406148
      Distribution of brain-derived neurotrophic factor in cranial and spinal ganglia.
      Zhou, X F, et al.
      Exp. Neurol., 149: 237-42 (1998)  1998

      Show Abstract
      9454633 9454633
      Endogenous brain-derived neurotrophic factor is anterogradely transported in primary sensory neurons.
      Zhou, X F and Rush, R A
      Neuroscience, 74: 945-53 (1996)  1996

      Show Abstract
      8895863 8895863
      A BDNF autocrine loop in adult sensory neurons prevents cell death.
      Acheson, A, et al.
      Nature, 374: 450-3 (1995)  1995

      7700353 7700353

      Data Sheet

      Title
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY

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      Alternative Format

      Catalog Number Description  
      AB1779 Anti-Brain Derived Neurotrophic Factor Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies