AB1779 Anti-Brain Derived Neurotrophic Factor Antibody

AB1779
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R WB, IHC, FUNC Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1779
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Brain Derived Neurotrophic Factor Antibody
      Alternate Names
      • BDNF
      References
      Product Information
      FormatSerum
      PresentationLyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water.
      Quality LevelMQ100
      Applications
      ApplicationDetect Brain Derived Neurotrophic Factor using this Anti-Brain Derived Neurotrophic Factor Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Affects Function
      Application NotesImmunohistochemistry: 1:1,000 (see suggested protocol).

      Immunoblotting:1:1,000; we recommend preparing the BDNF samples for western blot by the method of Katoh-Semba (1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C.

      BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant Human Brain Derived Neurotrophic Factor (BDNF).
      HostRabbit
      SpecificityBrain Derived Neurotrophic Factor (BDNF). By dot blot, less than 1% cross-reactivity against NGF, NT3 or NT4.

      SPECIES REACTIVITIES: Rat and human. Other species not yet tested. It is likely that the antibody will also work on mouse and porcine due to sequence homology.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
      Gene Symbol
      • BDNF
      • Abrineurin
      • neurotrophin
      • MGC34632
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
      SIZE: 247 amino acids; 27818 Da
      SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
      SUBCELLULAR LOCATION: Secreted.
      PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
      DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
      SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20 to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Brain Derived Neurotrophic Factor Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY - 23988442398844
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY - 24264622426462
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY - 24467332446733
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR - 25093582509358
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR -25088052508805
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY3099565
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY2913484
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY2976495
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY2999839
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY3020576

      References

      Reference overviewApplicationPub Med ID
      The role of brain-derived neurotrophic factor (BDNF) in the development of neurogenic detrusor overactivity (NDO).
      Frias, B; Santos, J; Morgado, M; Sousa, MM; Gray, SM; McCloskey, KD; Allen, S; Cruz, F; Cruz, CD
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  2146-60  2015

      Show Abstract
      25653370 25653370
      Electroacupuncture-like stimulation at Baihui and Dazhui acupoints exerts neuroprotective effects through activation of the brain-derived neurotrophic factor-mediated MEK1/2/ERK1/2/p90RSK/bad signaling pathway in mild transient focal cerebral ischemia in rats.
      Cheng, CY; Lin, JG; Su, SY; Tang, NY; Kao, ST; Hsieh, CL
      BMC complementary and alternative medicine  14  92  2014

      Show Abstract
      Western Blotting24606810 24606810
      A novel approach to induction and rehabilitation of deficits in forelimb function in a rat model of ischemic stroke.
      Livingston-Thomas, JM; Hume, AW; Doucette, TA; Tasker, RA
      Acta pharmacologica Sinica  34  104-12  2013

      Show Abstract
      23103624 23103624
      Transient domoic acid excitotoxicity increases BDNF expression and activates both MEK- and PKA-dependent neurogenesis in organotypic hippocampal slices.
      Pérez-Gómez, A; Tasker, RA
      BMC neuroscience  14  72  2013

      Show Abstract
      Immunohistochemistry23865384 23865384
      Different roles of BDNF in nucleus accumbens core versus shell during the incubation of cue-induced cocaine craving and its long-term maintenance.
      Li, X; DeJoseph, MR; Urban, JH; Bahi, A; Dreyer, JL; Meredith, GE; Ford, KA; Ferrario, CR; Loweth, JA; Wolf, ME
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  1130-42  2013

      Show Abstract
      Immunohistochemistry23325250 23325250
      Escalated or suppressed cocaine reward, tegmental BDNF, and accumbal dopamine caused by episodic versus continuous social stress in rats.
      Miczek, KA; Nikulina, EM; Shimamoto, A; Covington, HE
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  9848-57  2011

      Show Abstract
      21734276 21734276
      Roles of BDNF in spinal neuroplasticity in cats subjected to partial dorsal ganglionectomy.
      Rong Rong,Bu-Liang Meng,Nan Jiang,Li-Qun Hu,Ting-Hua Wang
      Growth factors (Chur, Switzerland)  29  2011

      Show Abstract
      21854347 21854347
      Cocaine-induced chromatin remodeling increases brain-derived neurotrophic factor transcription in the rat medial prefrontal cortex, which alters the reinforcing efficacy of cocaine.
      Sadri-Vakili, G; Kumaresan, V; Schmidt, HD; Famous, KR; Chawla, P; Vassoler, FM; Overland, RP; Xia, E; Bass, CE; Terwilliger, EF; Pierce, RC; Cha, JH
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  11735-44  2010

      Show Abstract Full Text Article
      20810894 20810894
      Immunolocalization of pro- and mature-brain derived neurotrophic factor (BDNF) and receptor TrkB in the human brainstem and hippocampus.
      Tang S, Machaalani R, Waters KA
      Brain Res  1354  1-14. Epub 2010 Jul 29.  2010

      Show Abstract
      20673758 20673758
      A switch in retrograde signaling from survival to stress in rapid-onset neurodegeneration.
      Perlson, E; Jeong, GB; Ross, JL; Dixit, R; Wallace, KE; Kalb, RG; Holzbaur, EL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  29  9903-17  2009

      Show Abstract Full Text Article
      19657041 19657041

      Data Sheet

      Title
      RABBIT ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies