AB1534 Anti-Brain Derived Neurotrophic Factor Antibody

AB1534
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H ELISA, IHC, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1534
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Brain Derived Neurotrophic Factor Antibody
      Alternate Names
      • brain-derived neurotrophic factor
      Background InformationBrain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.
      References
      Product Information
      FormatSerum
      Control
      • Brain tissue
      PresentationRabbit polyclonal antiserum in buffer containing no preservatives. Lyophilized. Reconstitute with 50 μL of sterile distilled water.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Brain Derived Neurotrophic Factor Antibody is validated for use in ELISA, IH, WB for the detection of Brain Derived Neurotrophic Factor.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Applications Not Recommended
      • Inhibits Activity/Function
      Application NotesImmunohistochemistry:
      1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).

      ELISA:
      1:1000-1:2000 dilution of a previous lot was used in ELISA.

      Bioassay:
      Does not inhibit BDNF biological activity.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityBrain Derived Neurotrophic Factor (BDNF). By dot blot the antibody shows less than 0.1% cross reactivity with recombinant human NT3, NT4/5 or mouse NGF.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
      Gene Symbol
      • BDNF
      • Abrineurin
      • neurotrophin
      • MGC34632
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
      SIZE: 247 amino acids; 27818 Da
      SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
      SUBCELLULAR LOCATION: Secreted.
      PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
      DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
      SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
      Molecular Weight18 kDa, with a homodimer at ~27-30 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on mouse brain lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Brain Derived Neurotrophic Factor Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Brain Derived Neurotrophic Factor Antibody Certificates of Analysis

      TitleLot Number
      Anti-Brain Derived Neurotrophic Factor2475583
      Anti-Brain Derived Neurotrophic Factor3073328
      Anti-Brain Derived Neurotrophic Factor - 21385462138546
      Anti-Brain Derived Neurotrophic Factor - 23970562397056
      Anti-Brain Derived Neurotrophic Factor - 20106452010645
      Anti-Brain Derived Neurotrophic Factor - 20326982032698
      Anti-Brain Derived Neurotrophic Factor - 20397692039769
      Anti-Brain Derived Neurotrophic Factor - 20491992049199
      Anti-Brain Derived Neurotrophic Factor - 20654152065415
      Anti-Brain Derived Neurotrophic Factor - 22192762219276

      References

      Reference overviewPub Med ID
      A role for the p75 neurotrophin receptor in axonal degeneration and apoptosis induced by oxidative stress.
      Kraemer, BR; Snow, JP; Vollbrecht, P; Pathak, A; Valentine, WM; Deutch, AY; Carter, BD
      The Journal of biological chemistry  289  21205-16  2014

      Show Abstract
      24939843 24939843
      Sex-dependent impacts of low-level lead exposure and prenatal stress on impulsive choice behavior and associated biochemical and neurochemical manifestations.
      Weston, HI; Weston, DD; Allen, JL; Cory-Slechta, DA
      Neurotoxicology  44  169-83  2014

      Show Abstract
      25010656 25010656
      Qualitative analysis of hippocampal plastic changes in rats with epilepsy supplemented with oral omega-3 fatty acids.
      Roberta M Cysneiros,Danuza Ferrari,Ricardo M Arida,Vera C Terra,Antonio-Carlos G de Almeida,Esper A Cavalheiro,Fulvio A Scorza
      Epilepsy & behavior : E&B  17  2010

      Show Abstract
      19969506 19969506
      Dynamic regulation of bHLH-PAS-type transcription factor NXF gene expression and neurotrophin dependent induction of the transcriptional control activity.
      Norihisa Ooe,Kentaro Kobayashi,Kozo Motonaga,Koichi Saito,Hideo Kaneko
      Biochemical and biophysical research communications  378  2009

      Show Abstract
      19083991 19083991
      Tapered progesterone withdrawal promotes long-term recovery following brain trauma.
      Sarah M Cutler, Jacob W Vanlandingham, Donald G Stein
      Experimental neurology  200  378-85  2006

      Show Abstract
      16797538 16797538
      Neuronal loss and expression of neurotrophic factors in a model of rat chronic compressive spinal cord injury.
      Kazuma Kasahara, Takao Nakagawa, Toshihiko Kubota, Kazuma Kasahara, Takao Nakagawa, Toshihiko Kubota
      Spine  31  2059-66  2006

      Show Abstract
      16915089 16915089
      Levels of brain-derived neurotrophic factor and neurotrophin-4 in lumbar motoneurons after low-thoracic spinal cord hemisection.
      Rosario Gulino, Salvatore Andrea Lombardo, Antonino Casabona, Giampiero Leanza, Vincenzo Perciavalle
      Brain research  1013  174-81  2004

      Show Abstract
      15193526 15193526
      Role of brain-derived neurotrophic factor in wobbler mouse motor neuron disease.
      K Tsuzaka, T Ishiyama, E P Pioro, H Mitsumoto, K Tsuzaka, T Ishiyama, E P Pioro, H Mitsumoto
      Muscle nerve  24  474-80  2001

      Show Abstract
      11268018 11268018

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies