AB1513P Anti-Brain Derived Neurotrophic Factor Antibody

AB1513P
500 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, R ELISA, IHC, FUNC, WB Sh Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1513P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Brain Derived Neurotrophic Factor Antibody
      Alternate Names
      • BDNF
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationProtein G Purified immunoglobulin. Lyophilized from PBS, no preservatives. Reconstitute with 500 μL of sterile distilled water.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, IH, FUNC & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Affects Function
      • Western Blotting
      Application NotesImmunohistochemistry: 1-5 μg/mL (see suggested protocol).

      Immunoblotting: 1-5 μg/mL we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochem. 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as ~12-18kDa monomer, with a 28-30kDa homodimer in most samples.

      ELISA: 1-5 μg/mL

      Inhibition of biological activity in vitro: Complete inhibition of BDNF activity on embryonic chick sensory neurons occurs at 1-5 μg/mL. Biological inhibition protocols can be found in Finn et al. (J. Neurocytol. 15:169-176, 1986).
      BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.

      Use neat for in vivo studies.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant Human Brain Derived Neurotrophic Factor (BDNF).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostSheep
      SpecificityRecognizes Brain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4. The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.
      Species Reactivity
      • Chicken
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
      Gene Symbol
      • BDNF
      • Abrineurin
      • neurotrophin
      • MGC34632
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
      SIZE: 247 amino acids; 27818 Da
      SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
      SUBCELLULAR LOCATION: Secreted.
      PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
      DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
      SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
      Molecular Weight~12-18kDa monomer, with a 28-30kDa homodimer in most samples.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Glycerol (1:1) can be added for additional stability. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Brain Derived Neurotrophic Factor Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Brain Derived Neurotrophic Factor Antibody Certificates of Analysis

      TitleLot Number
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR3170778
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR2470359
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY - 21158752115875
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY - 21657932165793
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY - 21506912150691
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) -31670083167008
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) - 33289983328998
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY3107383
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY2911581
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY3167587

      References

      Reference overviewPub Med ID
      Hippocampal--prefrontal BDNF and memory for fear extinction.
      Rosas-Vidal, LE; Do-Monte, FH; Sotres-Bayon, F; Quirk, GJ
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  39  2161-9  2014

      Show Abstract
      24625752 24625752
      Sexual differences in cell proliferation in the ventricular zone, cell migration and differentiation in the HVC of juvenile Bengalese finch.
      Chen, Q; Zhang, X; Zhao, Y; Zhou, X; Sun, L; Zeng, S; Zuo, M; Zhang, X
      PloS one  9  e97403  2014

      Show Abstract
      24841082 24841082
      Feedback mechanism in depolarization-induced sustained activation of extracellular signal-regulated kinase in the hippocampus.
      Maharana, C; Sharma, KP; Sharma, SK
      Scientific reports  3  1103  2013

      Show Abstract
      23346360 23346360
      Smilagenin attenuates beta amyloid (25-35)-induced degeneration of neuronal cells via stimulating the gene expression of brain-derived neurotrophic factor.
      R Zhang,Z Wang,P A Howson,Z Xia,S Zhou,E Wu,Y Hu
      Neuroscience  210  2012

      Show Abstract
      22441042 22441042
      Motoneuron programmed cell death in response to proBDNF.
      Taylor, AR; Gifondorwa, DJ; Robinson, MB; Strupe, JL; Prevette, D; Johnson, JE; Hempstead, B; Oppenheim, RW; Milligan, CE
      Developmental neurobiology  72  699-712  2012

      Show Abstract
      21834083 21834083
      Trk activation of the ERK1/2 kinase pathway stimulates intermediate chain phosphorylation and recruits cytoplasmic dynein to signaling endosomes for retrograde axonal transport.
      Mitchell, DJ; Blasier, KR; Jeffery, ED; Ross, MW; Pullikuth, AK; Suo, D; Park, J; Smiley, WR; Lo, KW; Shabanowitz, J; Deppmann, CD; Trinidad, JC; Hunt, DF; Catling, AD; Pfister, KK
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  15495-510  2012

      Show Abstract
      23115187 23115187
      Upregulation of CREB-mediated transcription enhances both short- and long-term memory.
      Suzuki, A; Fukushima, H; Mukawa, T; Toyoda, H; Wu, LJ; Zhao, MG; Xu, H; Shang, Y; Endoh, K; Iwamoto, T; Mamiya, N; Okano, E; Hasegawa, S; Mercaldo, V; Zhang, Y; Maeda, R; Ohta, M; Josselyn, SA; Zhuo, M; Kida, S
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  8786-802  2011

      Show Abstract
      21677163 21677163
      PSA-NCAM-dependent GDNF signaling limits neurodegeneration and epileptogenesis in temporal lobe epilepsy.
      Venceslas Duveau,Jean-Marc Fritschy
      The European journal of neuroscience  32  2010

      Show Abstract
      20597970 20597970
      Spinal alpha 2-adrenoceptor-mediated analgesia in neuropathic pain reflects brain-derived nerve growth factor and changes in spinal cholinergic neuronal function.
      Ken-ichiro Hayashida,James C Eisenach
      Anesthesiology  113  2010

      Show Abstract Full Text Article
      20613480 20613480
      Endogenous BDNF regulates induction of intrinsic neuronal growth programs in injured sensory neurons.
      Nicole M Geremia,Lina M E Pettersson,J C Hasmatali,Todd Hryciw,Nils Danielsen,David J Schreyer,Valerie M K Verge
      Experimental neurology  223  2010

      Show Abstract
      19646438 19646438

      Data Sheet

      Title
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies