AB1513 Anti-Brain Derived Neurotrophic Factor Antibody

AB1513
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R ELISA, WB, IHC, FUNC Sh Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1513
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Brain Derived Neurotrophic Factor Antibody
      Alternate Names
      • BDNF
      References
      Product Information
      FormatSerum
      PresentationSheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any insoluble material.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Brain Derived Neurotrophic Factor Antibody is an antibody against Brain Derived Neurotrophic Factor for use in ELISA, WB, IH.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      • Affects Function
      Application NotesImmunohistochemistry: 1:200-1:2,000 (see suggested protocol).

      Immunoblotting: 1:200-1:2,000 we recommend preparing the BDNF samples for western blot by the method of Semba-Katoh, R et.al (J. Neurochemistry 69(1):34-42, 1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH 7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C.

      ELISA: 1:200-1:2,000

      Inhibition of biological activity in vitro: 1:10-1:50

      Use neat for in vivo animal studies.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant Human Brain Derived Neurotrophic Factor (BDNF).
      HostSheep
      SpecificityBrain Derived Neurotrophic Factor (BDNF). Neutralizes BDNF, but not other neurotrophins. By one site ELISA, less than 1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4.

      The antibody was tested on human and rat derived samples, but based on sequence similarity it is expected to react with mouse and other mammalian species. The peptide sequences of human, rat and mouse BDNF are identical.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
      Gene Symbol
      • BDNF
      • Abrineurin
      • neurotrophin
      • MGC34632
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
      SIZE: 247 amino acids; 27818 Da
      SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
      SUBCELLULAR LOCATION: Secreted.
      PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
      DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
      SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Brain Derived Neurotrophic Factor Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Brain Derived Neurotrophic Factor Antibody Certificates of Analysis

      TitleLot Number
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY - 23877342387734
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY - 24332572433257
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR - 22110172211017
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR - 29538882953888
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR - 31821283182128
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR -26062682606268
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR -26070692607069
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR -27820942782094
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY3103602
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY2967962

      References

      Reference overviewPub Med ID
      MeCP2 controls BDNF expression and cocaine intake through homeostatic interactions with microRNA-212.
      Im, HI; Hollander, JA; Bali, P; Kenny, PJ
      Nature neuroscience  13  1120-7  2010

      Show Abstract
      20711185 20711185
      Nicotinic alpha 7 receptor clusters on hippocampal GABAergic neurons: regulation by synaptic activity and neurotrophins.
      Kawai, Hideki, et al.
      J. Neurosci., 22: 7903-12 (2002)  2002

      Show Abstract
      12223543 12223543
      Expression of brain-derived neurotrophic factor protein in the adult rat central nervous system
      Yan, Q, et al
      Neuroscience, 78:431-48 (1997)  1997

      9145800 9145800

      Data Sheet

      Title
      SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies