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AB9354 Anti-Beta III Tubulin Antibody

500 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IHC, WBChAffinity PurifiedPolyclonal Antibody
      Catalogue NumberAB9354
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Beta III Tubulin Antibody
      Background InformationTubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulin‎ is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue.
      Product Information
      FormatAffinity Purified
      • Brain tissue, Neuronal culture
      PresentationAffinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide.
      Four different antibodies were combined to make this product
      Quality LevelMQ100
      ApplicationAnti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells.

      1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues.

      Optimal working dilutions must be determined by the end user
      Biological Information
      ImmunogenSynthetic peptides from human/rat Beta III Tubulin.
      Epitope(Similar to TuJ1 antigen)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Beta III Tubulin
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • TUBB4
      • beta-4
      • MC1R
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight55 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot:
      1:1000 dilution of this lot detected TUBULIN BETA III on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information


      Anti-Beta III Tubulin Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Beta III Tubulin Antibody Certificates of Analysis

      TitleLot Number
      Anti-Beta III Tubulin - 2147014 2147014
      Anti-Beta III Tubulin - 2387758 2387758
      Anti-Beta III Tubulin - 2424655 2424655
      Anti-Beta III Tubulin - 2430359 2430359
      Anti-Beta III Tubulin - 2458974 2458974
      Anti-Beta III Tubulin - 2462756 2462756
      Anti-Beta III Tubulin - 1769107 1769107
      Anti-Beta III Tubulin - 1956386 1956386
      Anti-Beta III Tubulin - 1994663 1994663
      Anti-Beta III Tubulin - 2041550 2041550


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      Life Science Research > Antibodies and Assays > Primary Antibodies