AB9354 Anti-Beta III Tubulin Antibody

AB9354
500 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ICC, IHC, WB Ch Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB9354
      Replaces04-1049
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Beta III Tubulin Antibody
      Background InformationTubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulin‎ is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Brain tissue, Neuronal culture
      PresentationAffinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide.
      Four different antibodies were combined to make this product
      Quality LevelMQ100
      Applications
      ApplicationAnti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells.

      Immunohistochemistry:
      1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues.

      Optimal working dilutions must be determined by the end user
      Biological Information
      ImmunogenSynthetic peptides from human/rat Beta III Tubulin.
      Epitope(Similar to TuJ1 antigen)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostChicken
      SpecificityRecognizes Beta III Tubulin
      IsotypeIgY
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • TUBB4
      • beta-4
      • MC1R
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot:
      1:1000 dilution of this lot detected TUBULIN BETA III on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Beta III Tubulin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Beta III Tubulin Antibody Certificates of Analysis

      TitleLot Number
      Anti-Beta III Tubulin - 21470142147014
      Anti-Beta III Tubulin - 23877582387758
      Anti-Beta III Tubulin - 24246552424655
      Anti-Beta III Tubulin - 24303592430359
      Anti-Beta III Tubulin - 24589742458974
      Anti-Beta III Tubulin - 24627562462756
      Anti-Beta III Tubulin - 17691071769107
      Anti-Beta III Tubulin - 19563861956386
      Anti-Beta III Tubulin - 19946631994663
      Anti-Beta III Tubulin - 20415502041550

      References

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      Ferroni, L; Gardin, C; Sivolella, S; Brunello, G; Berengo, M; Piattelli, A; Bressan, E; Zavan, B
      International journal of molecular sciences  16  4666-81  2015

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      Cancer-associated TERT promoter mutations abrogate telomerase silencing.
      Chiba, K; Johnson, JZ; Vogan, JM; Wagner, T; Boyle, JM; Hockemeyer, D
      eLife  4  2015

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      Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery.
      Clauser, L; Ferroni, L; Gardin, C; Tieghi, R; Galiè, M; Elia, G; Piattelli, A; Pinton, P; Bressan, E; Zavan, B
      PloS one  9  e110796  2014

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      Immunohistochemistry25375632 25375632
      Cationic surface charge combined with either vitronectin or laminin dictates the evolution of human embryonic stem cells/microcarrier aggregates and cell growth in agitated cultures.
      Lam, AT; Li, J; Chen, AK; Reuveny, S; Oh, SK; Birch, WR
      Stem cells and development  23  1688-703  2014

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      Fluorescence Activated Cell Sorting (FACS)24641164 24641164
      Rapid neurogenesis through transcriptional activation in human stem cells.
      Busskamp, V; Lewis, NE; Guye, P; Ng, AH; Shipman, SL; Byrne, SM; Sanjana, NE; Murn, J; Li, Y; Li, S; Stadler, M; Weiss, R; Church, GM
      Molecular systems biology  10  760  2014

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      Enhanced synthesis and release of dopamine in transgenic mice with gain-of-function α6* nAChRs.
      Wang, Y; Lee, JW; Oh, G; Grady, SR; McIntosh, JM; Brunzell, DH; Cannon, JR; Drenan, RM
      Journal of neurochemistry  129  315-27  2014

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      Directing human induced pluripotent stem cells into a neurosensory lineage for auditory neuron replacement.
      Gunewardene, N; Bergen, NV; Crombie, D; Needham, K; Dottori, M; Nayagam, BA
      BioResearch open access  3  162-75  2014

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      LRIG2 mutations cause urofacial syndrome.
      Stuart, HM; Roberts, NA; Burgu, B; Daly, SB; Urquhart, JE; Bhaskar, S; Dickerson, JE; Mermerkaya, M; Silay, MS; Lewis, MA; Olondriz, MB; Gener, B; Beetz, C; Varga, RE; Gülpınar, O; Süer, E; Soygür, T; Ozçakar, ZB; Yalçınkaya, F; Kavaz, A; Bulum, B; Gücük, A; Yue, WW; Erdogan, F; Berry, A; Hanley, NA; McKenzie, EA; Hilton, EN; Woolf, AS; Newman, WG
      American journal of human genetics  92  259-64  2013

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      Anesthetics interfere with the polarization of developing cortical neurons.
      Mintz, CD; Smith, SC; Barrett, KM; Benson, DL
      Journal of neurosurgical anesthesiology  24  368-75  2012

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      Novel MeCP2 isoform-specific antibody reveals the endogenous MeCP2E1 expression in murine brain, primary neurons and astrocytes.
      Zachariah, RM; Olson, CO; Ezeonwuka, C; Rastegar, M
      PloS one  7  e49763  2012

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      23185431 23185431

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