OP91 Anti-Bcl-2 (Ab-3) Mouse mAb (4D7)

OP91
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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OP91-100UG
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      Plastic ampoule 100 μg
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      Description
      Overview

      This product has been discontinued.



      Recognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, breast carcinoma, and lymph node tissue.

      Catalogue NumberOP91
      Brand Family Calbiochem®
      References
      ReferencesLarsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
      Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Silverman, G.A., et al. 1993 Mol. Cell. Biol. 13, 5469.
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Vaux, D.L., 1993. Proc. Natl. Acad. Sci. USA 90, 786.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative controlBrain tissue
      Positive controlMCF-7 cells or breast carcinoma or lymph node tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation Tanaka, S., et al. 1993. J. Biol. Chem. 268, 10920. Immunofluorescence, Flow Cytometry Aiello, O., et al. 1992. Cytometry 13, 502. Immunoblotting Reed, J.C., et al. 1990. Anal. Biochem. 205, 70. Flow Cytometry Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.
      Key Applications Immunoprecipitation
      Paraffin Sections
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesFlow Cytometry (see application references)
      Immunoblotting (2 µg/ml, see application references)
      Immunofluorescence (see application references)
      Immunoprecipitation (see application references)
      Paraffin Sections (1 µg/ml)
      Application CommentsOnly random cells will stain for bcl-2 in brain tissue. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (ASRDPVARTSPLQTPA) corresponding to amino acids 61-76 of human Bcl-2
      ImmunogenHuman
      Clone4D7
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Bcl-2 (Ab-3) Mouse mAb (4D7) Certificates of Analysis

      TitleLot Number
      OP91

      References

      Reference overview
      Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
      Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Silverman, G.A., et al. 1993 Mol. Cell. Biol. 13, 5469.
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Vaux, D.L., 1993. Proc. Natl. Acad. Sci. USA 90, 786.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.

      Citations

      Title
    • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      ApplicationFlow Cytometry (see application references)
      Immunoblotting (2 µg/ml, see application references)
      Immunofluorescence (see application references)
      Immunoprecipitation (see application references)
      Paraffin Sections (1 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse SP2/0 cells. Recognizes the ~24 kDa Bcl-2 protein.
      BackgroundThe bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a ~24-26 kDa protein which has been localized to the inner mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death." It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock and irradiation.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (ASRDPVARTSPLQTPA) corresponding to amino acids 61-76 of human Bcl-2
      Clone4D7
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlMCF-7 cells or breast carcinoma or lymph node tissue
      Negative controlBrain tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsOnly random cells will stain for bcl-2 in brain tissue. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesLarsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
      Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Silverman, G.A., et al. 1993 Mol. Cell. Biol. 13, 5469.
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Vaux, D.L., 1993. Proc. Natl. Acad. Sci. USA 90, 786.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
      Citation
    • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
    • Application referencesImmunoprecipitation Tanaka, S., et al. 1993. J. Biol. Chem. 268, 10920. Immunofluorescence, Flow Cytometry Aiello, O., et al. 1992. Cytometry 13, 502. Immunoblotting Reed, J.C., et al. 1990. Anal. Biochem. 205, 70. Flow Cytometry Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.