GF35L Sigma-AldrichAnti-BDNF Mouse mAb (35928.11)

Anti-BDNF, mouse monoclonal, clone 35928.11, recognizes the ~20 kDa BDNF protein in brain extracts. It is validated for ELISA, Western blotting, immunohistochemistry, and neutralization studies.

Anti-BDNF Mouse mAb (35928.11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Synonyms: Anti-Brain Derived Neurotrophic Factor

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Overview

Replacement Information

Key Specifications Table

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

Pricing & Availability

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Description
Overview	Recognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
Catalogue Number	GF35L
Brand Family	Calbiochem®
Synonyms	Anti-Brain Derived Neurotrophic Factor

References
References	Riccio, A., et al. 1997. Science 277 1097. Sendtner, M., et al. 1996. Neurochem. Res. 21, 831. Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442. Gotz, R., et al. 1994. Nature 372, 266. Snider, W.D. 1994. Cell 77, 627. Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.

Product Information
Form	Lyophilized
Formulation	Lyophilized from PBS, 5% trehalose.
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunohistochemistry Neutralization Studies
Application Notes	ELISA (0.5-1 µg/ml) Immunoblotting (1-2 µg/ml) Immunohistochemistry (5-15 µg/ml) Neutralization Studies (see comments)
Application Comments	The full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant protein consisting of amino acids 129-247 of human BDNF
Immunogen	Human
Clone	35928.11
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Documentation

Anti-BDNF Mouse mAb (35928.11) SDS

Title
Safety Data Sheet (SDS)

Anti-BDNF Mouse mAb (35928.11) Certificates of Analysis

Title	Lot Number
GF35L	Enter Lot Number

References

Reference overview
Riccio, A., et al. 1997. Science 277 1097. Sendtner, M., et al. 1996. Neurochem. Res. 21, 831. Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442. Gotz, R., et al. 1994. Nature 372, 266. Snider, W.D. 1994. Cell 77, 627. Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	12-April-2010 JSW
Synonyms	Anti-Brain Derived Neurotrophic Factor
Application	ELISA (0.5-1 µg/ml) Immunoblotting (1-2 µg/ml) Immunohistochemistry (5-15 µg/ml) Neutralization Studies (see comments)
Description	Immunoaffinty purified mouse monoclonal antibody. Recognizes the ~20 kDa BDNF protein.
Background	Neurotrophic factors define a family of related proteins, which are important in the development, maintenance, and regulation of neurons in both the peripheral and central nervous system. In addition to the prototypical nerve growth factor (NGF), several other factors have been identified which interact with a family of tyrosine kinase receptors. TrkA is the kinase signaling receptor for NGF, while TrkB mediates signaling for both brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5). TrkC is the primary receptor for neurotrophin-3 (NT-3), although this neurotrophin can also activate TrkA and TrkB receptors under certain conditions. Axotomy-induced cell death is one experimental paradigm that has been used to discriminate between the effects of various neurotrophic factors on the survival of different neuronal populations. BDNF, NT-3, and NT-4/5 act as motor neuron survival factors, whereas NGF has no effect on the survival of spinal cord motor neurons. NGF is neuroprotective for cholinergic neurons in the central nervous system. Retrogradely transported NGF-TrkA has also been shown to signal the CREB transcription factor in sympathetic neurons. A putative new member of this gene family, neurotrophin-6 (NT-6), has also been identified from the fish Xephophorus. Like NGF, NT-6 promotes the survival of sympathetic and sensory neurons, albeit with a lower potency.
Host	Mouse
Immunogen species	Human
Immunogen	recombinant protein consisting of amino acids 129-247 of human BDNF
Clone	35928.11
Isotype	IgG₁
Species	human
Form	Lyophilized
Formulation	Lyophilized from PBS, 5% trehalose.
Preservative	None
Comments	The full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Riccio, A., et al. 1997. Science 277 1097. Sendtner, M., et al. 1996. Neurochem. Res. 21, 831. Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442. Gotz, R., et al. 1994. Nature 372, 266. Snider, W.D. 1994. Cell 77, 627. Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.