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AB3594-50UL Anti-Aquaporin 4 Antibody, CT

AB3594-50UL
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3594-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Aquaporin 4 Antibody, CT
      Alternate Names
      • AQP4
      • WCH4
      • Mercurial-Insensitive Water Channel
      Background InformationWater is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity Purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Aquaporin 4 Antibody, C-terminus is validated for use in IH, WB for the detection of Aquaporin 4.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blotting: 1:500 on rat brain membranes.

      Immunohistochemistry

      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863). It is expected that the antibody may also react with mouse (73/75), bovine (71/75), human (69/75) and rabbit (64/75) due to sequence homology.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes aquaporin 4. On western blots of rat brain membranes, the antibody detects a band migrating at approximately 38 kDa corresponding to aquaporin 4.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • AQP4
      • MIWC
      • WCH4
      • AQP-4
      • aquaporin-4
      • HMIWC2
      • Aquaporin-4
      • MGC22454
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55087 # Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
      SIZE: 323 amino acids; 34830 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea.
      DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
      SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
      Molecular Weight38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Aquaporin 4 Antibody, CT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Aquaporin 4 Antibody, CT Certificates of Analysis

      TitleLot Number
      -004RABBIT ANTI-AQUAPORIN 4 AFFINITY PURIFED POLYCLONAL ANTIBODY 2938927
      RABBIT ANTI-AQUAPORIN 4 AFFINITY PURIFED POLYCLONAL ANTIBODY - 2390397 2390397
      RABBIT ANTI-AQUAPORIN 4 AFFINITY PURIFED POLYCLONAL ANTIBODY - 2453131 2453131
      RABBIT ANTI-AQUAPORIN 4 - 2908049 2908049
      RABBIT ANTI-AQUAPORIN 4 - 3209132 3209132
      RABBIT ANTI-AQUAPORIN 4 - 3226287 3226287
      RABBIT ANTI-AQUAPORIN 4 - 3257095 3257095
      RABBIT ANTI-AQUAPORIN 4 - 3286087 3286087
      RABBIT ANTI-AQUAPORIN 4 - 3398584 3398584
      RABBIT ANTI-AQUAPORIN 4 - 3434541 3434541

      Data Sheet

      Title
      RABBIT ANTI-AQUAPORIN 4 AFFINITY PURIFED POLYCLONAL ANTIBODY

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies