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AB3594-200UL Anti-Aquaporin 4 Antibody, CT

AB3594-200UL
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIHC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3594-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Aquaporin 4 Antibody, CT
      Alternate Names
      • Mercurial-Insensitive Water Channel
      Background InformationWater is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.
      References
      Product Information
      FormatPurified
      Control
      • Included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationPurified rabbit polyclonal lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationDetect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was used on rat brain sections.
      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Western blotting:
      1:500-1:1,000 using ECL on rat brain membranes.

      Optimal working dilutions must be determined by the end user
      Biological Information
      ImmunogenGST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, mouse, and rat. Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • AQP4
      • MIWC
      • WCH4
      • AQP-4
      • HMIWC2
      • MGC22454
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P55087 # Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
      SIZE: 323 amino acids; 34830 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea.
      DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
      SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. ...
      Molecular Weight38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.
      Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Aquaporin 4 Antibody, CT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Aquaporin 4 Antibody, CT Certificates of Analysis

      TitleLot Number
      Anti-Aquaporin 4, C-terminus 2465122
      Anti-Aquaporin 4, C-terminus 3070792
      Anti-Aquaporin 4, C-terminus 3072342
      Anti-Aquaporin 4, C-terminus - 2383052 2383052
      Anti-Aquaporin 4, C-terminus - 2453113 2453113
      Anti-Aquaporin 4, C-terminus - 1959570 1959570
      Anti-Aquaporin 4, C-terminus - 1987447 1987447
      Anti-Aquaporin 4, C-terminus - 2015536 2015536
      Anti-Aquaporin 4, C-terminus - 2036065 2036065
      Anti-Aquaporin 4, C-terminus - 2089946 2089946

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies